miR-130b inhibits proliferation and promotes differentiation in myocytes via targeting Sp1  

作　　者：Yu-Cheng Wang Xiaohan Yao Mei Ma Huihui Zhang Hui Wang Lei Zhao Shengnan Liu Chao Sun Peng Li Yuting Wu Xihua Li Jingjing Jiang Yuying Li Yan Li Hao Ying 

机构地区：[1]Shanghai Xuhui District Central Hospital,Zhongshan-Xuhui Hospital,Fudan University,Shanghai 200001,China [2]CAS Key laboratory of Nutrition,Metabolism and Food Safety,Shanghai Institute of Nutrition and Health,Chinese Academy of Sciences,Shanghai 200031,China [3]Department of Neuromuscular Disease,Children’s Hospital of Fudan University,Shanghai 201102,China [4]Department of Endocrinology and Metabolism,Zhongshan Hospital,Fudan University,Shanghai 200031,China [5]State Key Laboratory of Food Science and Technology,School of Food Science and Technology,Jiangnan University,Wuxi 214122,China [6]Key Laboratory of Food Safety Risk Assessment,Ministry of Health,Beijing 100021,China

出　　处：《Journal of Molecular Cell Biology》2021年第6期422-432,共11页分子细胞生物学报（英文版）

基　　金：This work was supported by grants from the National Natural Science Foundation of China(81570768 to Y.-C.W.,31900841 to Yan Li,91957205 and 31525012 to H.Y.,and 81471016 to J.J.);the Ministry of Science and Technology of China(2016YFA0500102 and 2016YFC1304905);CAS Key Laboratory of Nutrition,Metabolism and Food Safety(KLNMFS2019-01),and Chinese Academy of Sciences Interdisciplinary Innovation Team.

摘　　要：Muscle regeneration after damage or during myopathies requires a fine cooperation between myoblast proliferation and myogenic differentiation.A growing body of evidence suggests that microRNAs play critical roles in myocyte proliferation and differentiation transcriptionally.However,the molecular mechanisms underlying the orchestration are not fully understood.Here,we showed that miR-130b is able to repress myoblast proliferation and promote myogenic differentiation via targeting Sp1 transcription factor.Importantly,overexpression of miR-130b is capable of improving the recovery of damaged muscle in a freeze injury model.Moreover,miR-130b expression is declined in the muscle of muscular dystrophy patients.Thus,these results indicated that miR-130b may play a role in skeletal muscle regeneration and myopathy progression.Together,our findings suggest that the miR-130b/Sp1 axis may serve as a potential therapeutic target for the treatment of patients with muscle damage or severe myopathies.

关 键 词：miR-130b muscle regeneration PROLIFERATION DIFFERENTIATION SP1 

分 类 号：R685[医药卫生—骨科学]