茶饼病诱导感、抗茶树品种的基因表达谱分析  

Gene Expression Profiling Analysis of Resistant and Susceptible Tea Cultivars in Response to Tea Blister Blight

作　　者：龙亚芹[1] 冉隆珣[1] 夏丽飞[1] 曲浩 田易萍[1] 陈林波[1] 梁名志[1] Long Yaqin;Ran Longxun;Xia Lifei;Qu Hao;Tian Yiping;Chen Linbo;Liang Mingzhi(Yunnan Provincial Key Laboratory of Tea Science,Yunnan Engineering Research Center of Tea Germplasm Innovation and Matching Cultivation,Yunnan Province Academy of Agricultaral Sciences Institute of Tea,Menghai,666201)

机构地区：[1]云南省农业科学院茶叶研究所,云南省茶树种质资源创新与配套栽培技术工程研究中心,云南省茶学重点实验室,勐海666201

出　　处：《分子植物育种》2021年第18期6020-6034,共15页Molecular Plant Breeding

基　　金：国家自然科学基金项目(No.31660224);国家重点研发项目(2016YFD0200903);国家茶叶产业技术体系(CARS-19);云南省人才培养计划项目(2015HB105)共同资助。

摘　　要：为揭示茶树被茶饼病危害诱导的防御反应机制,本研究选择抗病和感病茶树品种为材料,通过转录组测序和数字表达谱分析茶树叶片被茶饼病危害前后的基因表达差异。结果显示,共筛选出差异表达基因974条,其中共有的为122条,抗病品种中特异364条,感病品种中特异的为488条。对差异表达基因进行分析发现,茶饼病危害主要影响了茶树体内代谢途径、内质网蛋白质加工、次生代谢产物的生物合成、植物病原相互作用、植物激素信号转导途径、淀粉与蔗糖的代谢、苯丙醇生物合成等通路中关键基因的表达水平,这些差异基因包括抗病蛋白基因(R protein)、水解酶基因、细胞壁加固基因、转录因子基因、植物激素及其信号转导基因、次生代谢和氧化酶类、转运蛋白等。利用RT-qPCR对筛选的6个基因进行验证,其表达模式与测序结果一致。本研究初步明确了茶饼病侵染对茶树基因转录水平的影响,为揭示茶树抗病的分子机制奠定了基础。In or der to reveal the defense response mechanism of tea plants induced by tea blister blight, we selected resistant and s usceptible tea plants as materials, and analyzed the gene expression differences before and after tea leaves were damaged by tea blister blight by transcriptome sequencing and digital expression profile.The results showed a total of 974 DEGs were identified, of which 122 DEGs were co-expressed genes in both resistant and susceptible cultivars, 364 DEGs were specific genes only in blister disease-resistant cultivar, and 488 DEGs were specific genes only in blight disease-susceptible cultivar. The infection of tea blister blight mainly affected the expression levels of key genes involved in metabolic pathways, protein processing in endoplasmic reticulum,biosynthesis of secondary metabolites, plant-pathogen interaction, plant hormone signal transduction, starch and sucrose metabolism, phenylalanine, tyrosine and tryptophan biosynthesis and so on. These DEGs included disease resistance protein gene, hydrolase gene, cell wall reinforcement genes, transcription factor genes, plant hormones and their signal transduction genes, second ary metabolism and oxidase genes, transporter genes and so on. The differential expression s of 6 genes were verified by quantitative Real-time PCR, which showed a general consistency consistent with the results of transcriptome sequencing. This study preliminarily clarified the influence of tea blister blight infection on gene transcription levels in tea plants with this study, which laid a theoretical foundation for researching the molecular mechanism of disease resistance in tea plants.

关 键 词：茶树 茶饼病 转录组测序 抗病基因 

分 类 号：S435.711[农业科学—农业昆虫与害虫防治]