吉林地区沙棘红茶活性成分及抗氧化活性的研究  

Study on the Chemical Constituents and Antioxidant Activity of Black Tea from Hipopphae Rhamnoides L.Leaves in Jilin Province

作　　者：冉丽梅 周鸿立[1] 刘宇琦 刘莹 RAN Limei;ZHOU Hongli;LIU Yuqi;LIU Ying(School of Chemistry and Pharmaceutical Engineering,Jilin Institute of Chemical Technology,Jilin 132022,China)

机构地区：[1]吉林化工学院化学与制药工程学院,吉林吉林132022

出　　处：《吉林化工学院学报》2021年第9期5-8,52,共5页Journal of Jilin Institute of Chemical Technology

摘　　要：目的:对沙棘叶及其制品红茶进行化学成分的研究,并对其抗氧化活性进行评价.方法:利用福林酚比色法、亚硝酸钠-硝酸铝-氢氧化钠显色法、苯酚硫酸法和国家标准方法《茶咖啡碱测定》,对沙棘茶中茶多酚、黄酮、多糖和咖啡碱含量进行分析;采用DPPH·、ABTS+、OH·和还原力4种方法进行抗氧化评价.结果:沙棘红茶主要化学成分:茶多酚为18.45%,黄酮含量为2.51%,多糖含量为15.56%,咖啡碱含量为3.06%;体外抗氧化实验结果表明,沙棘叶及其制品红茶对清除DPPH·、ABTS+、·OH自由基的IC50分别为2.47和0.76 mg·mL^(-1)、0.0040和0.0038 mg·mL^(-1)、0.48和0.41 mg·mL^(-1),还原力与浓度有较好的剂量依赖关系.结论:由于沙棘红茶中含有有茶多酚、黄酮和多糖等活性成分因而有较好的抗氧化活性.The chemical constituents and antioxidant potentials of Hipopphae rhamnoides L.leaves and its black tea product were investigated.Methods:The contents of tea polyphenols,flavonoids,polysaccharides and caffeine were analyzed by Folin colorimetric method,Sodium nitrite-aluminum nitrate-sodium hydroxide colorimetric method,Phenol-sulfuric acid method and national standard method of“Theophylline determination”;Four methods including DPPH·,ABTS+,·OH and reducing power were used to evaluated antioxidant activity.Results:The main chemical components of the black tea:tea polyphenols was 18.45%,flavonoids content was 2.51%,polysaccharides content was 15.56%and caffeine content was 3.06%;the results of in vitro antioxidant experiments showed that the IC50 of Hipopphae rhamnoides L.leaves and its black tea on scavenging DPPH·,ABTS+and·OH free radicals were 2.47 and 0.76 mg·mL^(-1),0.0040 and 0.0038 mg·mL^(-1),0.48 and 0.41 mg·mL^(-1),respectively.They also had a certain reducing power and a good concentration dependence.Conclusions:The antioxidant activity of black tea is better because of active ingredients such as tea polyphenols,flavonoids and polysaccharides.

关 键 词：沙棘茶 茶多酚 抗氧化活性 

分 类 号：S793.6[农业科学—林木遗传育种]