苦荞黄酮含量与SSR标记的关联分析  

Correlation Between Flavonoids Content and SSR Markers of Tartary Buckwheat

作　　者：石桃雄[1] 黎瑞源[1] 潘凡[1] 黄娟[1] 朱丽伟[1] 汪燕[1] 梁成刚[1] SHI Taoxiong;LI Ruiyuan;PAN Fan;HUANG Juan;ZHU Liwei;WANG Yan;LIANG Chenggang(Buckwheat Industry Technology Research Center,Guizhou Normal University,Guiyang,Guizhou 550001,China)

机构地区：[1]贵州师范大学荞麦产业技术研究中心,贵州贵阳550001

出　　处：《福建农业学报》2021年第8期884-891,共8页Fujian Journal of Agricultural Sciences

基　　金：国家重点研发计划项目(2019YFD1001300,2019YFD1001301);国家自然科学基金项目(31960125);贵州省科技支撑计划项目(黔科合ZC[2019]2298);贵州省荞麦种质资源保育及创新重点实验室建设基金项目(黔教合KY[2017]002);贵州师范大学博士启动基金项目(11904/0514027)。

摘　　要：【目的】开展苦荞籽粒总黄酮含量与SSR标记的关联分析,挖掘与黄酮含量相关的分子标记,为高黄酮含量苦荞品种的遗传改良提供依据。【方法】以193份苦荞种质为供试材料,基于62对SSR引物分析了供试种质的遗传多样性,对总黄酮含量和SSR标记进行了关联分析。【结果】193个种质籽粒总黄酮含量的变幅为1.04%~2.99%,变异系数为27.93%。62对引物在193个种质中共扩增出267个等位基因,每个SSR位点平均检测到2.45个有效等位基因,基因多样性为0.503,Shannon信息指数为0.942,观测杂合度为0.513,引物多态信息量为0.74。群体结构分析将193份种质划分为3个亚群。基于广义线性模型(GLM)共检测到5个与籽粒总黄酮含量显著关联的SSR标记,表型贡献率为6.3%~12.9%,其中标记TatG0124(12.9%)和S6853(8.5%)的表型贡献率较高。【结论】供试苦荞种质遗传多样性丰富,可用于苦荞重要农艺和品质性状的关联分析。TatG0124和SSR6853可能是控制籽粒黄酮含量的重要位点,对改良苦荞籽粒黄酮含量具有重要意义。【Objective】Correlation between the flavonoids content and associated markers was analyzed for genetic improvement studies on Tartary buckwheat.【Method】Sixty-two SSR primer pairs were used to assess the genetic diversity of 193 Tartary buckwheat germplasms.The SSR markers closely related to the total flavonoids content were selected by association analysis.【Result】The total flavonoids content of 193 Tartary buckwheat germplasms varied from 1.04%to 2.99%with a coefficient variation of 27.93%.Sixty-two pairs of SSR primers amplified 267 polymorphic sites with an average number of effective alleles of 2.45.The mean values of gene diversity,Shannon index,observed heterozygosity and polymorphism information content of SSR markers were 0.503,0.942,0.513 and 0.74,respectively.The population structure analysis divided the 193 germplasms into 3 subgroups.Five SSR markers were determined to be significantly associated with flavonoids content by the general linear model(GLM).The phenotypic variations ranged from 6.3%to 12.9%with TatG0124 at 12.9%and S6853 at 8.5%being the higher markers.【Conclusion】The germplasms used in this study were diverse in genetic variation and feasible for related genome-wide mapping on important traits of Tartary buckwheat.The loci,TatG0124 and S6853,appeared to closely associate with the flavonoids content of Tartary buckwheat and were considered to be potentially useful for breeding new varieties with high content of the functional components.

关 键 词：苦荞 SSR标记 黄酮含量 遗传多样性 群体结构 关联分析 

分 类 号：S517[农业科学—作物学]