Celastrol induces lipophagy via the LXRα/ABCA1 pathway in clear cell renal cell carcinoma  

作　　者：Chan-juan Zhang Neng Zhu Jia Long Hong-tao Wu Yu-xiang Wang Bi-yuan Liu Duan-fang Liao Li Qin 

机构地区：[1]Division of Stem Cell Regulation and Application,School of Pharmacy,Hunan University of Chinese Medicine,Changsha 410208,China [2]Department of Urology,The First Hospital of Hunan University of Chinese Medicine,Changsha 410007,China [3]Department of Urology,The Second XiangYa Hospital of Central South University,Changsha 410011,China [4]Department of Immunology,School of Medicine,Hunan University of Chinese Medicine,Changsha 410208,China

出　　处：《Acta Pharmacologica Sinica》2021年第9期1472-1485,共14页中国药理学报（英文版）

基　　金：the National Natural Science Foundation of China(No.81973668,No.81774130 and No.81270359);the Natural Science Foundation of Hunan Province for Distinguished Young Scholars(No.2018JJ1018);the First-Class Discipline of Pharmaceutical Science of Hunan.

摘　　要：Celastrol is a triterpene derived from the traditional Chinese medicine Tripterygium wilfordii Hook f,which displays potential anticancer activity.In the present study,we investigated the anticancer effects of celastrol against clear cell renal cell carcinoma(ccRCC)and the underlying mechanisms.Using Cancer Genome Atlas(TCGA)database and genotype-tissue expression(GTEx)database we conducted a bioinformatics analysis,which showed that the mRNA levels of liver-X receptorsα(LXRα)and ATP-binding cassette transporter A1(ABCA1)in ccRCC tissues were significantly lower than those in adjacent normal tissues.This result was confirmed by immunoblotting analysis of 4 ccRCC clinical specimens,which showed that the protein expression of LXRαand ABCA1 was downregulated.Similar results were obtained in a panel of ccRCC cell lines(786-O,A498,SN12C,and OS-RC-2).In 786-O and SN12C cells,treatment with celastrol(0.25–2.0μM)concentration-dependently inhibited the cell proliferation,migration,and invasion as well as the epithelial-mesenchymal transition(EMT)process.Furthermore,we demonstrated that celastrol inhibited the invasion of 786-O cells through reducing lipid accumulation;celastrol concentration-dependently promoted autophagy to reduce lipid storage.Moreover,we revealed that celastrol dramatically activated LXRαsignaling,and degraded lipid droplets by inducing lipophagy in 786-O cells.Finally,celastrol promoted cholesterol efflux from 786-O cells via ABCA1.In high-fat diet-promoted ccRCC cell line 786-O xenograft model,administration of celastrol(0.25,0.5,1.0 mg·kg^(−1)·d^(−1),for 4 weeks,i.p.)dose-dependently inhibited the tumor growth with upregulated LXRαand ABCA1 protein in tumor tissue.In conclusion,this study reveals that celastrol triggers lipophagy in ccRCC by activating LXRα,promotes ABCA1-mediated cholesterol efflux,suppresses EMT progress,and ultimately inhibits cell proliferation,migration,and invasion as well as tumor growth.Thus,our study provides evidence that celastrol can be used as a lipid met

关 键 词：clear cell renal cell carcinoma CELASTROL lipophagy LXRΑ ABCA1 epithelial-mesenchymal transition 

分 类 号：R363[医药卫生—病理学]