利用转录组分析蓝光对金针菇原基形成的影响  

Transcriptome Analysis Revealed Effect of Blue Light on Primordium Formation of Flammulina filiformis

作　　者：刘建雨 王瑞娟[1] 张丹[1] 徐珍[1] 陆欢[1] 宋春艳[1] 尚晓冬[1] 于海龙[1] LIU Jianyu;WANG Ruijuan;ZHANG Dan;XU Zhen;LU Huan;SONG Chunyan;SHANG Xiaodong;YU Hailong(Institute of Edible Fungi,Shanghai Academy of Agricultural Sciences,Key Laboratory of Edible Fungi Resources and Utilization(South),Ministry of Agriculture and Rural Affairs,P.R.China,National Engineering Research Center of Edible Fungi,Shanghai Key Laboratory of Agricultural Genetics and Breeding,Shanghai 201403,China)

机构地区：[1]上海市农业科学院食用菌研究所,农业农村部应用真菌资源与利用重点实验室,国家食用菌工程技术研究中心,上海市农业遗传育种重点实验室,上海201403

出　　处：《食用菌学报》2021年第5期29-36,共8页Acta Edulis Fungi

基　　金：上海市科技兴农项目[沪农科创字(2020)第1-3号]。

摘　　要：按照工厂化生产模式栽培金针菇(Flammulina filiformis)菌株T022,在搔菌后第3天给予蓝光照射,每天6 h,照射3 d,以黑暗处理为对照,利用转录组分析研究蓝光对金针菇原基形成的影响。结果显示:蓝光组的原基数量增多,气生菌丝减少;蓝光组与黑暗组原基的差异表达基因有168个,其中上调基因76个,下调基因92个。差异基因的GO功能富集分析显示,蓝光主要影响原基中与细胞壁成分相关的疏水蛋白以及位于细胞膜的转运蛋白基因的表达量。蓝光组与对照组相比,与细胞壁相关的7个疏水蛋白基因表达量下调;与细胞膜相关的6个转运蛋白基因表达量上调,15个转运蛋白基因表达量下调。Primordial stage is very important for the development of Flammulina filiformis.Using darkness as the control,the industrial cultivation strain T022 was treated with blue light 2 d after scratching for three consecutive days with 6 h every day.The results showed that the number of primordial buds increased and the number of aerial hypha decreased in the blue light group.There were 168 differential genes between the blue light group and the control group.Among the differential genes,76 were up-regulated and 92 were down-regulated.GO enrichment analysis showed that blue light mainly effected the expression of primordial hydrophobins associated with cell wall components and transmembrane proteins.Compared with the control,seven hydrophobin genes pertaining to cell wall components were down-regulated,six cell membrane related protein genes were up-regulated,and 15 transporter genes were down-regulated.

关 键 词：金针菇 蓝光 原基 疏水蛋白 转运蛋白 

分 类 号：S646.15[农业科学—蔬菜学]