塔罗科血橙室温贮藏期间花色苷和糖酸积累变化及相关代谢基因表达特征  

Changes of anthocyanin,sugar and acid accumulation and expression characteristics of related metabolic genes in Tarocco blood oranges during room temperature storage

作　　者：洪敏 贺明阳[1,2,3] 王日葵 周炼[1,2] 王晶[1,2] 冯雨 HONG Min;HE Mingyang;WANG Rikui;ZHOU Lian;WANG Jing;FENG Yu(Citrus Research Institute,Southwest University,Chongqing 400712,China;Citrus Research Institute,Chinese Academy of Agricultural Sciences,Chongqing 400712,China;Key Laboratory of Plant Resources Conservation and Sustainable Utilization,South China Botanical Garden,Chinese Academy of Sciences,Guangzhou 510650,China)

机构地区：[1]西南大学柑桔研究所,重庆400712 [2]中国农业科学院柑桔研究所,重庆400712 [3]中国科学院华南植物园植物资源保护与可持续利用重点实验室,广州510650

出　　处：《浙江大学学报（农业与生命科学版）》2021年第5期589-597,共9页Journal of Zhejiang University:Agriculture and Life Sciences

基　　金：重庆市自然科学基金(cstc2019jcyj-msxmX0690,cstc2018jcyjAX0256);中央高校基本科研业务费专项资金(XDJK2020C027);中国博士后科学基金(2018M633178)。

摘　　要：以塔罗科血橙为试验材料,运用高效液相色谱和实时荧光定量聚合酶链反应技术,分析室温贮藏期间果实花色苷和糖酸含量的变化规律及相关代谢基因表达特征。结果表明:贮藏期间,血橙花色苷含量提高了222%,花色苷积累与4CL、CHI、ANS表达水平呈显著正相关。总糖、蔗糖、果糖和葡萄糖含量分别提高了12.0%、7.7%、19.7%、12.7%,蔗糖积累与SS1表达水平呈显著正相关,与AI呈极显著负相关。柠檬酸含量呈显著的波动变化,柠檬酸积累与IDH表达水平呈极显著负相关。4CL、CHI、ANS、SS1、AI、IDH在花色苷和糖酸代谢过程中发挥重要作用。整个贮藏期间,血橙花色苷积累与总糖、蔗糖、果糖和葡萄糖含量呈显著正相关,且与蔗糖相关性最高。综上所述,短期室温贮藏可提高塔罗科血橙花色苷和可溶性糖含量,可溶性糖积累是促进血橙采后花色苷合成的重要因素。Using Tarocco blood oranges as experimental materials,the changes of anthocyanin,soluble sugar and organic acid contents and the expression characteristics involved in related metabolic genes during room temperature storage were analyzed by high performance liquid chromatography(HPLC)and quantitative real time polymerase chain reaction.The results showed as follows:During room temperature storage,the anthocyanin content of blood oranges was increased by 222%.The mRNA levels of 4CL,CHI and ANS were significantly positive correlation with the anthocyanin content.The total sugar,sucrose,fructose and glucose contents of the fruits were significantly promoted by 12.0%,7.7%,19.7%and 12.7%,respectively.The mRNA level of SS1 was significantly positive correlation with the sucrose content,and the mRNA level of AI was extremely significant and negative correlation with the sucrose content.The accumulation of citric acid fluctuated significantly,and the mRNA level of IDH was extremely significant and negative correlation with the citric acid content.The 4CL,CHI,ANS,SS1,AI and IDH genes played important roles in the metabolism of anthocyanin,sugar and acid.Correlation analysis indicated that the anthocyanin content in blood oranges was significantly positive correlation with the contents of total sugar,sucrose,fructose,and glucose during room temperature storage,which had the highest correlation with the sucrose content.The above results show that the anthocyanin and soluble sugar contents of Tarocco blood oranges are promoted by short-term room temperature storage,and the changes of soluble sugar accumulation may regulate the anthocyanin synthesis.

关 键 词：塔罗科血橙 花色苷 可溶性糖 有机酸 表达分析 相关性分析 

分 类 号：S379.2[农业科学—农产品加工] S666[农业科学—农艺学]