铁皮石斛提取物与红茶提取物联用改善切除卵巢致骨质疏松小鼠骨组织形态的研究  

Effects of the Combination of Dendrobium candidum Extract and Black Tea Extract on Improving Bone Morphology in Ovariectomized Osteoporosis Mice

作　　者：王明月 孙泽锐 安孟飞 申畅 胡财江 赵云丽 王宣军[1,4] 盛军 WANG Mingyue;SUN Zerui;AN Mengfei;SHEN Chang;HU Caijiang;ZHAO Yunli;WANG Xuanjun;SHENG Jun(Key Laboratory of Pu-erh Tea Science,Ministry of Education,Yunnan Agricultural University,Kunming 650201,China;College of Food Science and Technology,Yunnan Agricultural University,Kunming 650201,China;Key Laboratory of Medicinal Chemistry for Natural Resource,Ministry of Education,Yunnan Provincial Center for Research&Development of Natural Products,School of Chemical Science and Technology,Yunnan University,Kunming 650091,China;College of Science,Yunnan Agricultural University,Kunming 650201,China)

机构地区：[1]云南农业大学,普洱茶学教育部重点实验室,云南昆明650201 [2]云南农业大学食品科学技术学院,云南昆明650201 [3]云南大学化学科学与工程学院,教育部自然资源药物化学重点实验室,云南省天然产物转化与应用重点实验室,云南昆明650091 [4]云南农业大学理学院,云南昆明650201

出　　处：《云南农业大学学报（自然科学版）》2021年第5期855-861,918,共8页Journal of Yunnan Agricultural University:Natural Science

基　　金：云南省科技厅基金项目[2018IA060,2017ZF003,2017FG001(-046),2017FD084];国家自然科学基金项目(31960075,21602196)。

摘　　要：【目的】研究铁皮石斛提取物与红茶提取物联用对切除卵巢小鼠骨组织形态参数的影响。【方法】建立骨质疏松症小鼠模型,并将小鼠随机分成6组,分别为假手术组(S组,未切除卵巢)、模型组(M组)、雌二醇组(E2组,1.5 mg/kg)以及铁皮石斛与红茶提取物联用低剂量组(DCE+BTE-L组,0.475 g/kg)、中剂量组(DCE+BTE-M组,0.950 g/kg)和高剂量组(DCE+BTE-H组,1.425 g/kg);连续给予受试物3个月后,用Micro-CT对骨质疏松模型小鼠的左侧股骨进行形态计量学参数分析。【结果】铁皮石斛提取物与红茶提取物联用处理小鼠可改善骨小梁结构,增加骨密度(BMD)、骨小梁体积分数(BV/TV)和骨小梁厚度(Tb.Th),并降低骨小梁分离度(Tb.Sp),其中DCE+BTE-M组与M相比效果最佳,可明显改善骨小梁结构,且BMD值增加约2倍(P<0.05),BV/TV增长约1倍。【结论】铁皮石斛提取物与红茶提取物联用处理骨质疏松模型小鼠可明显改善切除卵巢导致的骨微结构退变。[Purpose]To study the effect of the combination of Dendrodium candidum extract and black tea extract on bone morphometric parameters in ovariectomized mice.[Methods]A mouse model of osteoporosis was established,and those mice were randomly divided into six groups,including a sham operation group(S group,unexcised ovary),a model group(M group),an estradiol group(E2 group,1.5 mg/kg),the combination of D.candidum extract and black tea extract low dose group(DCE+BTE-L group,0.475 g/kg),medium dose group(DCE+BTE-M group,0.950 g/kg)and high dose group(DCE+BTE-H,1.425 g/kg).After three months of continuous administration of the test substances,the left femur morphometric parameters of osteoporosis model mice was analyzed by Micro-CT.[Results]The combination of D.candidum extract and black tea extract treated mice could improve bone trabecular structure,increase bone mineral density(BMD),bone volume/total volume(BV/TV)and bone trabecular thickness(Tb.Th),as well as decrease bone trabecular separation(Tb.Sp);among them,compared with the M group,the DCE+BTE-M group had the best effect,which obviously improved the trabecular bone structure,and BMD values increased by about two times(P<0.05),BV/TV increased by about one time.[Conclusion]Treatment of osteoporosis model mice with the combination of D.candidum extract and black tea extract obviously improved bone microstructural degeneration induced by ovariectomy.

关 键 词：铁皮石斛 红茶 提取物 骨质疏松症 骨微结构 

分 类 号：S571.101[农业科学—茶叶生产加工] S682.31[农业科学—作物学]