基于高通量测序分析果桑茶对2型糖尿病模型小鼠肠道菌群的影响  

Effect of fruit mulberry tea on intestinal microbiota of type 2 diabetic mice based on high-throughput sequencing

作　　者：魏建敏 杨华连 陈莉[1,2] 卢红梅 石庆叠[1,2] 张祥瑞 涂青 WEI Jianmin;YANG Hualian;CHEN Li;LU Hongmei;SHI Qingdie;ZHANG Xiangrui;TU Qing(Guizhou Key Laboratory of Fermentation Engineering and Biopharmacy,Guiyang 550025,China;School of Liquor and Food Engineering,Guizhou University,Guiyang 550025,China)

机构地区：[1]贵州省发酵工程与生物制药重点实验室,贵州贵阳550025 [2]贵州大学酿酒与食品工程学院,贵州贵阳550025

出　　处：《食品与发酵工业》2021年第20期75-82,共8页Food and Fermentation Industries

基　　金：贵州省科技支撑计划项目(黔科合支撑[2019]2371号)。

摘　　要：为研究果桑茶对2型糖尿病模型小鼠肠道菌群的调节作用,利用高脂饮食联合链脲佐菌素诱导构建2型糖尿病小鼠模型,收集不同组别小鼠粪便进行高通量测序,分析其菌群变化情况。结果表明,2型糖尿病的产生会导致小鼠肠道菌群失调,其中模型对照组小鼠肠道菌群多样性最低,其部分有益菌相对丰度降低,有害菌相对丰度升高。与之相比,果桑茶茶粉剂量组(GDm组)和果桑茶水提物剂量组(GDh组)显著增加了Muribaculaceae_unclassified、艾克曼菌属(Akkermansia)、毛螺菌科(Lachnospiraceae)等有益菌群的丰度,丰富了菌群多样性,其中GDm组肠道菌群与正常对照组最接近,调节效果最为显著。该研究证明了果桑茶能有效调节2型糖尿病模型小鼠肠道菌群结构和丰度,促进肠道菌群良性发展,为果桑降糖保健产品的开发提供理论依据。In order to study the regulatory effect of fruit mulberry tea on intestinal microbiota in type 2 diabetes mice model,high-fat diet combined with streptozotocin was used to induce the type 2 diabetic mice model.The feces of mice from different groups were collected to analyze the changes in the microbiota using high-throughput sequencing.The results showed that the occurrence of type 2 diabetes could lead to the imbalance of intestinal microbiota in mice,among which the diversity of intestinal microbiota in the control group was the lowest,the relative abundance of some beneficial bacteria was decreased,and the relative abundance of harmful bacteria was increased.In contrast,fruit mulberry tea powder dose group(GDm group)and fruit mulberry tea extract dose group(GDh group)significantly increased the relative abundance of Muribaculaceae_unclassified,Akkermansia,Lachnospiraceae and other beneficial bacteria,enriching the diversity of bacteria.The intestinal microbiota of the GDm group was the closest to that of the normal control group,and the regulation effect was the most significant.This research proves that fruit mulberry tea can effectively regulate the structure and abundance of intestinal microbiota in type 2 diabetes model mice,promote the benign development of intestinal microbiota,providing theoretical basis for the development of fruit mulberry hypoglycemic health products.

关 键 词：果桑茶 2型糖尿病 肠道菌群 菌群分析 高通量测序 

分 类 号：TS272.5[农业科学—茶叶生产加工] R587.1[轻工技术与工程—农产品加工及贮藏工程]