响应面法优化漆籽油提取工艺及其抗氧化活性研究  

Optimization of extraction technology of lacquer seed oil by response surface methodology and its antioxidant activity

作　　者：周昊[1,2] 薛兴颖 叶建中 王成章[1,2] ZHOU Hao;XUE Xingying;YE Jianzhong;WANG Chengzhang(Jiangsu Key Lab.of Biomass Energy and Material,Key Lab.of Chemical Engineering of Forest Products of National Forestry and Grassland Administration,National Engineering Lab.for Biomass Chemical Utilization,Institute of Chemical Industry of Forest Products,Chinese Academy of Forestry,Nanjing 210042,China;Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources,Nanjing Forestry University,Nanjing 210037,China)

机构地区：[1]中国林业科学研究院林产化学工业研究所,生物质化学利用国家工程实验室,国家林业和草原局林产化学工程重点实验室,江苏省生物质能源与材料重点实验室,南京210042 [2]南京林业大学江苏省林业资源高效加工利用协同创新中心,南京210037

出　　处：《中国油脂》2021年第9期5-10,共6页China Oils and Fats

基　　金：中国林科院基本科研业务费专项资金(CAFYBB2018ZB009)。

摘　　要：以秦巴山区漆籽为原料,采用溶剂(石油醚)浸提法从其种仁中提取漆籽油,在单因素试验的基础上,采用响应面法优化漆籽油提取工艺,对漆籽油的理化性质进行分析,采用气质联用分析漆籽油的脂肪酸组成,并评价其抗氧化活性。结果表明:漆籽油的最优提取工艺条件为提取温度70℃、提取时间60 min、液料比10∶1、提取次数2次,在此条件下漆籽油提取率为96.14%;漆籽油相对密度为0.925 8,折光指数(20℃)为1.467 2,碘值(I)为146.83 g/100 g,酸值(KOH)为2.58 mg/g,过氧化值为3.62 mmol/kg,皂化值(KOH)为185.36 mg/g;漆籽油主要含有油酸、亚油酸、棕榈酸、硬脂酸、亚麻酸和棕榈油酸6种脂肪酸,不饱和脂肪酸含量达86.10%,以油酸和亚油酸为主,二者含量达到82.17%;漆籽油对DPPH、ABTS及羟自由基的半数清除浓度(IC_(50))分别为35.38、41.29、38.52 mg/L,具有与维生素C相当的抗氧化活性。Lacquer seed oil was extracted from kernel of lacquer seeds from Qinba Mountain by solvent(petroleum ether) extraction.On the basis of single factor experiment, response surface methodology was used to optimize the extraction process of lacquer seed oil. The physicochemical indexes of lacquer seed oil were analyzed, and the fatty acid composition of lacquer seed oil was analyzed by GC-MS, its antioxidant activity was also evaluated. The results showed that the optimal extraction process of lacquer seed oil were obtained as follows: extraction temperature 70℃, extraction time 60 min, liquid-material ratio 10∶1, extraction times twice.Under the optimal conditions, the extraction rate of lacquer seed oil was 96.14%.The relative density, refractive index(20℃), iodine value, acid value, peroxide value and saponification value of lacquer seed oil were 0.925 8, 1.467 2, 146.83 gI/100 g, 2.58 mgKOH/g, 3.62 mmol/kg and 185.36 mgKOH/g, respectively. Lacquer seed oil mainly contained six kinds of fatty acids including oleic acid, linoleic acid, palmitic acid, stearic acid, linolenic acid and palmitoleic acid, and the content of unsaturated fatty acids reached up to 86.10%, in which the content of oleic acid and linoleic acid reached 82.17%. The IC_(50) of lacquer seed oil for DPPH, ABTS and hydroxyl radicals were 35.38, 41.29 mg/L and 38.52 mg/L, showing equivalent antioxidant activity with vitamin C.

关 键 词：漆籽油 溶剂提取 响应面优化 理化性质 脂肪酸组成 抗氧化活性 

分 类 号：TQ645.1[化学工程—精细化工] O657.65[理学—分析化学]