A widespread response of Gram-negative bacterial acyl-homoserine lactone receptors to Gram-positive Streptomyces γ-butyrolactone signaling molecules  

作　　者：Xiang Liu Wenxi Wang Junyue Li Yue Li Jihui Zhang Huarong Tan 

机构地区：[1]State Key Laboratory of Microbial Resources,Institute of Microbiology,Chinese Academy of Sciences,Beijing 100101,China [2]College of Life Sciences,University of Chinese Academy of Sciences,Beijing 100049,China

出　　处：《Science China(Life Sciences)》2021年第10期1575-1589,共15页中国科学（生命科学英文版）

基　　金：supported by the National Key Research and Development Program of China(2018YFA0901900 and 2020YFA0907700);the National Natural Science Foundation of China(31771378 and 31800029)。

摘　　要：Cell-cell communication is critical for bacterial survival in natural habitats,in which miscellaneous regulatory networks are encompassed.However,elucidating the interaction networks of a microbial community has been hindered by the population complexity.This study reveals thatγ-butyrolactone(GBL)molecules from Streptomyces species,the major antibiotic producers,can directly bind to the acyl-homoserine lactone(AHL)receptor of Chromobacterium violaceum and influence violacein production controlled by the quorum sensing(QS)system.Subsequently,the widespread responses of more Gram-negative bacterial AHL receptors to Gram-positive Streptomyces signaling molecules are unveiled.Based on the cross-talk between GBL and AHL signaling systems,combinatorial regulatory circuits(CRC)are designed and proved to be workable in Escherichia coli(E.coli).It is significant that the QS systems of Gram-positive and Gram-negative bacteria can be bridged via native Streptomyces signaling molecules.These findings pave a new path for unlocking the comprehensive cell-cell communications in microbial communities and facilitate the exploitation of innovative regulatory elements for synthetic biology.

关 键 词：signaling communication CROSS-TALK Gram-positive bacteria STREPTOMYCES Γ-BUTYROLACTONE Gram-negative bacteria acyl-homoserine lactone synthetic biology combinatorial regulatory circuit 

分 类 号：Q936[生物学—微生物学]