Tangshenping granule inhibits pyroptosis in a rat model of streptozotocin-induced diabetic nephropathy via the NLRP3/caspase-1/GSDMD pathway  

作　　者：Kun Gao Pengfei Zheng Tao Yang Xinxue Zhang Zongjiang Zhao 

机构地区：[1]School of Traditional Chinese Medicine,Beijing University of Chinese Medicine,Beijing,102488,China

出　　处：《Journal of Traditional Chinese Medical Sciences》2021年第4期317-326,共10页中医科学杂志（英文）

基　　金：supported by the National Natural Science Foundation of China(81373831,30973831)。

摘　　要：Objective: To explore the inhibitory effect of Tangshenping(TSP) on pyroptosis in a streptozotocininduced diabetic nephropathy(DN) rat model.Methods: DN was established in Spraguee Dawley rats. Rats were randomly divided into DN(model group), irbesartan, and TSP low-, medium-, and high-dose groups, besides the control group. The 24 h albuminuria content, and serum content of TC, TGs, Scr, IL-1 b, UREA, LDLs, and IL-18 were assessed.Hematoxylin & eosin and Mallory staining were performed to examine pathological changes in the kidney. The mRNA and protein expression of NLRP3, caspase 1, and GSDMD in the kidney were also examined.Results: The 24 h albuminuria content was obviously lower in the treatment groups compared to the model group(all P <.01). Levels of TC, TGs, Scr, UREA, LDLs, and IL-18 after drug interventions were obviously lower compared to the model group(all P <.05). The serum content of IL-1 b in the TSP medium-and high-dose groups were much lower compared to the model group(P =.013 and P=.001,respectively). Through immunohistochemistry and western blotting, we observed that the protein expressions of NLRP3, caspase-1, GSDMD, IL-1 b, and IL-18 were lower after drug interventions compared to the model group(all P <.05). Using qPCR, we observed that the mRNA expressions of caspase-1, IL-1 b, IL-18, and GSDMD after drug interventions were significantly lower compared to the model group(all P <.05). The m RNA expressions of NLRP3 in the TSP medium-and high-dose groups were both lower compared to the model group(all P <.05).Conclusion: TSP downregulated mRNA and protein expressions of NLRP3, caspase-1, and GSDMD. Our findings demonstrate that the beneficial effects of TSP on renal function are at least partly mediated by the inhibition of micro-inflammation and modulation of the expression of pyroptosis-related factors.

关 键 词：Tangshenping PYROPTOSIS Diabetic nephropathy STREPTOZOTOCIN 

分 类 号：R285.5[医药卫生—中药学]