不同贮藏温度对黄瓜花粉生活力的影响  

Effect of Different Storage Temperatures on Viability of Cucumber Pollen

作　　者：洪嘉骏 牛欢欢 李征[1] HONG Jia-jun;NIU Huan-huan;LI Zheng(College of Horticulture,Northwest A&F Univeristy,Yangling,Shaanxi 712100)

机构地区：[1]西北农林科技大学园艺学院,陕西杨凌712100

出　　处：《安徽农业科学》2021年第20期57-60,共4页Journal of Anhui Agricultural Sciences

摘　　要：首先对黄瓜花粉生活力的鉴定方法进行分析,随后对花粉管萌发检测方法进行了优化,包括最适蔗糖浓度、最适培养观察时间的筛选。结果表明不同蔗糖浓度的液体培养基对花粉生活力影响很大,以15%蔗糖+100 mg/kg硼酸效果最佳。培养时间方面,以培养1.5 h后观察花粉萌发状态最佳。随后,分析贮藏温度对黄瓜花粉生活力的影响。结果表明较于常温(25℃恒温贮藏)和-20℃恒温贮藏,4℃恒温贮藏能有效减缓花粉活性的下降速度。最后,比较了自交系与杂交商品种在不同贮藏条件下的花粉生活力。结果表明商品种相较于供试自交系,在相同贮藏条件下花粉萌发率较高,表明黄瓜花粉活性可能具有杂种优势效应。The identification methods of cucumber pollen viability were compared and analyzed, and then the detection methods of pollen tube germination were optimized, including the selection of the optimal sucrose concentration and the optimal culture observation time. The results showed that the liquid medium with different sucrose concentration had a great influence on pollen viability, and 15% sucrose + 100 mg/kg boric acid was an optimal choice. In terms of culture time, pollen germination was the best after 1.5 h. Then, the effect of storage temperature on the viability of cucumber pollen was analyzed. The results showed that compared with normal temperature(25 ℃ storage) and-20 ℃ storage, 4 ℃ storage could effectively slow down the decline of pollen activity. Finally, the pollen viability of inbred lines and commercial hybrids under different storage conditions was compared. The results showed that the pollen germination rate of commercial varieties was higher than that of the tested inbred lines under the same storage conditions, indicating that the pollen activity of cucumber may have heterosis effect.

关 键 词：黄瓜花粉 花粉生活力 花粉管萌发检测法 贮藏温度 

分 类 号：S642.2[农业科学—蔬菜学]