海洋降解麻痹性贝类毒素细菌的筛选及其耐毒能力初探  

Screening of Marine Bacteria Degrading and Tolerating Paralytic Shellfish Toxins

作　　者：张庆芳[1,2] 韩鹏飞 谢丹丹 迟雪梅 迟乃玉[1,2] 刘春莹 ZHANG Qingfang;HAN Pengfei;XIE Dandan;CHI Xuemei;CHI Naiyu;LIU Chunying(College of Life Science and Technology,Dalian University,Dalian 116622,Liaoning China;Liaoning Marine Microbial Engineering Technology Research Center,Dalian 116622,Liaoning China)

机构地区：[1]大连大学生命科学与技术学院,辽宁大连116622 [2]辽宁省海洋微生物工程技术研究中心,辽宁大连116622

出　　处：《饲料博览》2021年第10期5-10,共6页Feed Review

基　　金：国家重点研发计划项目(2019YFC0312600)。

摘　　要：麻痹性贝类毒素(PSTs)由藻类产生,能够富集在贝类体内,引起人类中毒,对我国食品、饲料行业等产生极大威胁。为了得到能够降解麻痹性贝类毒素的海洋菌株,通过平板涂布筛选方法,从野生牡蛎消化腺中筛选得到3株可能具有降解N-磺酰氨甲酰基类毒素能力的菌株(命名为H-2、H-3、H-4),绘制其在0、0.301、0.750 mol·L^(-1)和15.050 mol·L^(-1) N-磺酰氨甲酰基类毒素影响下的生长曲线,分析各菌株耐毒或降解毒素的能力。结果表明:H-3号菌株能够利用、降解N-磺酰氨甲酰基类毒素;H-2、H-4号菌株具有耐N-磺酰氨甲酰基类毒素的能力。通过形态学鉴定,初步判断这3株菌为细菌,N-磺酰氨甲酰基类毒素具有使其细胞形态均一化、缩小的影响。Paralytic shellfish toxins are produced by algae and can be enriched in shellfish,causing shellfish and human poisoning,which poses a great threat to the safety of China's food and fodder industry.In order to obtain marine strains capable of degrading shellfish poison,a plate coating method was used to screen the bacteria from the digestive glands of wild oysters.Three strains(named H-2,H-3 and H-4)were screened,which might be capable of degrading N-sulfonyl carbamoyl toxoid.Three strains growth curves under the influence of 0,0.301,0.750 mol·L^(-1) and 15.050 mol·L^(-1) concentrations of N-sulfonyl carbamoyl toxins were drawn to analyze the drug resistance or toxin degradation ability of each strain.The results showed that H-3 strain could degrade N-sulfonyl carbamoyl toxins by using strains H-2 and H-4 which were resistant to N-sulfonyl carbamoyl toxins.Through morphological identification,it was preliminarily judged that the three strains were bacteria,and N-sulfonyl carbamoyl toxins had the effect of homogenizing the cell morphology and reducing the cell size.

关 键 词：N-磺酰氨甲酰基类毒素 生长曲线 降解毒素 海洋细菌 水产养殖 

分 类 号：S852.44[农业科学—基础兽医学]