大孔树脂纯化裸花紫珠总三萜工艺研究  

Purification of the Total Triterpenes Extracted from Callicarpa nudiflora Hook.et Arn.by Macroporous Resins

作　　者：柯春山 吴少福[1] 彭鹏 李麟 李书飞 洪艳平[1] KE Chunshan;WU Shaofu;PENG Peng;LI lin;LI Shufei;HONG Yanping(College of Food Science and Engineering,Jiangxi Key Laboratory of Natural Products and Functional Food,Nanchang 330045,China;College of Animal Science and Technology,Jiangxi Agricultural University,Jiangxi Key Laboratory of Animal Disease Diagnosis and Control,Nanchang 330045,China)

机构地区：[1]江西农业大学食品科学与工程学院/江西省天然产物与功能食品重点实验室,江西南昌330045 [2]江西农业大学动物科学技术学院/江西省畜禽疫病诊断与防控重点实验室,江西南昌330045

出　　处：《江西农业大学学报》2021年第5期1178-1187,共10页Acta Agriculturae Universitatis Jiangxiensis

基　　金：江西省自然科学基金项目(20192BAB204022)。

摘　　要：【目的】从多种大孔树脂中优选出纯化裸花紫珠总三萜的最适工作树脂,研究最适工作树脂AB-8纯化裸花紫珠总三萜的最佳工艺条件。【方法】通过测试静态条件下树脂的吸附率和解吸率,从多种树脂中筛选出最适工作树脂;以总三萜浓度为指标,通过静态和动态吸附-解吸试验,优选出纯化裸花紫珠总三萜的最佳工艺条件。【结果】筛选出AB-8为最适工作树脂,纯化裸花紫珠总三萜的最佳工艺条件:(1)静态吸附:吸附pH 5、吸附温度35℃、吸附时间8 h;(2)静态解吸:洗脱时间4 h、洗脱温度55℃、pH 2的无水乙醇;(3)动态吸附:浓度1.5 mg/mL 40%的乙醇溶液、流速3 mL/min;(4)动态解吸:流速3 mL/min的无水乙醇洗脱。【结论】在最佳工艺条件下静态纯化所得固形物总三萜含量(28.11%)较纯化前提高了3.96倍,动态纯化所得总三萜含量(31.33%)较纯化前提高了4.42倍。[Objective]The optimum resin for purifying total triterpenoids from Callicarpa nudiflora Hook.et Arn.was selected from kinds of resins,and the optimum purification conditions for total triterpenoids,which derived from callicarpa nudiflora Hook.et Arn.through AB-8 resin,were investigated.[Method]The optimum operating resin was chosen from kinds of resins based on the static adsorption rate and static desorption rate of total triterpenoids.Utilizing the concentration of total triterpenes as reference,the optimal static/dynamic adsorption-desorption conditions for purification of total triterpenes were achieved[.Result]Among of the resins,AB-8 was determined as the optimum operating resin,the best conditions were as follows:(1)static adsorption:pH was 5,temperature was 35℃,adsorption time was 8 h;(2)static desorption:desorption time was 4 h,desorption temperature was 55℃,and anhydrous alcohol with pH 2;(3)dynamic adsorption:concentration of the sample solution(Vethanol∶Vwater=2∶3)was 1.5 mg/mL,velocity of flow was 3 mL/min;(4)dynamic desorption:anhydrous alcohol at the flow rate of 3 mL/min[.Conclusion]Under the gained conditions,the percentage of total triterpenes(28.11%)obtained by static purification was 3.96 times higher than that before purification.Further,the percentage of total triterpenes(31.33%)obtained by dynamic purification was 4.42 times higher than that before purification.

关 键 词：裸花紫珠 总三萜 大孔树脂 纯化 

分 类 号：R284.2[医药卫生—中药学]