Chrysanthemum indicum ethanol extract attenuates hepatic stellate cell activation in vitro and thioacetamide-induced hepatofibrosis in rats  

作　　者：Yun-Jin Chae Sushruta Koppula Myong-Ki Kim Tony Yoon MinDong Song 

机构地区：[1]Department of Applied Life Science,Graduate School of Konkuk University,268 Chungwon-daero,Chungju-si,Chungbuk,27478,Republic of Korea [2]Department of Biotechnology,College of Biomedical and Health Sciences,Konkuk University,268 Chungwon-daero,Chungju-si,Chungbuk,27478,Republic of Korea [3]Research Institute and College of Biomedical&Health Science,Konkuk University,268 Chungwon-daero,Chungju-si,Chungbuk,27478,Republic of Korea [4]Scholar Foxtrot Co.,LTD,501,SinGwan-dong 5F,Jongam-ro 36-gil,Seongbuk-gu,Seoul,02796,Republic of Korea [5]Department of Food Science and Engineering,Seowon University,Cheongju,Chungbuk 28674,Republic of Korea [6]Food One Corp,127,Sincheoksandan 5-ro,Deoksan-eup,Jincheon-gun,Chungbuk 27850,Republic of Korea

出　　处：《Asian Pacific Journal of Tropical Biomedicine》2021年第11期500-509,共10页亚太热带生物医学杂志（英文版）

基　　金：supported by Konkuk University in 2020。

摘　　要：Objective:To investigate the antifibrotic effects of Chrysanthemum indicum ethanol extract(CIEE)against activated hepatic stellate cells(HSC)and thioacetamide(TAA)-induced hepatofibrosis in rats.Methods:Cell viability and proliferation of HSC-T6 cells were measured using MTT assay.Primary HSCs were used to study morphology.TAA(200 mg/kg)was used to induced hepatic fibrosis in rats.CIEE(100 and 500 mg/kg)and silymarin(50 mg/kg)were administered orally.Liver functions including alanine transaminase,aspartate transaminase,glutathione,and hydroxyproline levels were measured using commercial kits.Liver sections and fibrotic biomarker expression were measured using hematoxylin and eosin staining and real-time polymerase chain reaction.Results:In vitro study revealed that CIEE(0.1,0.25,and 0.5 mg/mL)inhibited the proliferation of activated HSCs exposed to transforming growth factor(TGF)-β and restored the activated primary HSC morphology.In in vivo studies,TAA-induced increase in liver/body weight ratio(5.46±0.26)was significantly reduced(4.13±0.22)by CIEE(P<0.05 at 500 mg/kg).CIEE(100 and 500 mg/kg)improved the liver functions by significantly attenuating changes in alanine transaminase,aspartate transaminase,glutathione,and hydroxyproline levels(P<0.05).Further,CIEE(100 and 500 mg/kg)ameliorated the histological changes in liver tissue and TGF-β expression significantly(P<0.05)in TAA-induced rats.Conclusions:CIEE significantly protects against TAA-induced liver damage in rats and can be used in the treatment of liver fibrosis.

关 键 词：Chrysanthemum indicum Fibrosis HEPATOPROTECTIVE HYDROXYPROLINE THIOACETAMIDE 

分 类 号：R285.5[医药卫生—中药学]