基于网络药理学的大建中汤治疗胃癌的作用机制分析  

Action mechanism of Dajianzhong Decoction for gastric cancer based on network pharmacology

作　　者：陈思宇 陈姗 成沛玉[3] 阳帆 Chen Siyu;Chen Shan;Cheng Peiyu(School of Nursing,University of South China,Hengyang,Hunan 421001,China)

机构地区：[1]南华大学护理学院,湖南衡阳421001 [2]绍兴市人民医院,浙江绍兴312000 [3]湘潭市第一人民医院,湖南湘潭411104

出　　处：《湘南学院学报（医学版）》2021年第3期6-12,共7页Journal of Xiangnan University（Medical Sciences）

摘　　要：目的运用网络药理学的方法探讨大建中汤治疗胃癌的作用机制,为后期实验研究提供理论依据。方法通过中药系统药理学分析平台(TCMSP)对大建中汤的活性成分和靶点进行筛选;利用GeneCards、OMIM、PharmGKB和Therapeutic Target Database数据库获取胃癌靶点。利用R语言将药物与疾病靶点进行并集分析,获得疾病与药物共同靶点信息。将共同靶点导入CytoscaPe3.8.2,构建“药物—疾病—靶点”网络图,再利用STRING平台构建蛋白质相互作用网络。最后利用R语言对共同靶点进行基因本体(GO)分析和基因相互作用(KEGG)通路分析。结果筛选出大建中汤有效活性成分30个,大建中汤与胃癌的共同靶点101个,大建中汤主要通过调控AKT1,IL-6,TP53,TNF,VEGFA等靶基因及TNF信号通路、IL-17信号通路、HIF-1信号通路等发挥治疗胃癌的作用。结论大建中汤可通过多成分、多靶点、多通路治疗胃癌。Objective The action mechanism of Dajianzhong Decoction on gastric cancer was discussed by using the method of network pharmacology to provide theoretical basis for later experimental research.Methods The active ingredients and their targets of Dajianzhong Decoction were screened through the Traditional Chinese Medicine Systems Pharmacology Database(TCMSP),and gastric cancer targets were obtained from GeneCards database,OMIM database,PharmGKB,and Therapeutic Target Database.The combination analysis of drug and disease target is carried out by using R language to obtain the information of common targets of disease and drug.The common targets were introduced into CytoscaPe3.8.2 to construct a“drug-disease-target”network diagram,and the protein interaction network was constructed based on STRING platform.Finally,R language was used for gene ontology(GO)analysis and gene interaction(KEGG)pathway analysis for common targets.Results In Dajianzhong Decoction,30 active ingredients and 101 common targets were screened out.Dajianzhong Decoction mainly plays a role in the treatment of gastric cancer by regulating AKT1,IL-6,TP53,TNF,VEGFA and other target genes as well as TNF signaling pathway,IL-17 signaling pathway and HIF-1 signaling pathway.Conclusion Dajianzhong Decoction can treat gastric cancer through multiple components,multiple targets and multiple pathways.

关 键 词：大建中汤 胃癌 网络 药理学 药物作用机制 

分 类 号：R735.2[医药卫生—肿瘤]