L-cysteine-modified chiral gold nanoparticles promote periodontal tissue regeneration  

作　　者：Shuang Zhang Hong Zhou Na Kong Zezheng Wang Huangmei Fu Yangheng Zhang Yin Xiao Wenrong Yang Fuhua Yan 

机构地区：[1]Nanjing Stomatological Hospital,Medical School of Nanjing University,Nanjing,Jiangsu,People’s Republic of China [2]Key Laboratory of Optic-electric Sensing and Analytical Chemistry for Life Science,MOE,College of Chemistry and Molecular Engineering,Qingdao University of Science and Technology,Qingdao,266042,People’s Republic of China [3]School of Life and Environmental Science,Centre for Chemistry and Biotechnology,Deakin University,Geelong,VIC,Australia [4]Institute of Health and Biomedical Innovation,Queensland University of Technology,Brisbane,Queensland,4059,Australia [5]Australia-China Centre for Tissue Engineering and Regenerative Medicine,Queensland University of Technology,Australia

出　　处：《Bioactive Materials》2021年第10期3288-3299,共12页生物活性材料（英文）

基　　金：This work was supported by The National Natural Science Foundation Project[grant numbers 81771078,81570982,21675074,21675075];Jiangsu Provincial Medical Innovation Team,The Project of Invigorating Health Care through Science,Technology and Education[grant number CXTDB2017014];the Nanjing Clinical Research Center for Oral Diseases[grant number 2019060009];The Natural Science Foundation of Shandong Province(ZR2018ZC0231).

摘　　要：Gold nanoparticles(AuNPs)with surface-anchored molecules present tremendous potential in tissue regeneration.However,little is known about chiral-modified AuNPs.In this study,we successfully prepared L/D-cysteine-anchored AuNPs(L/D-Cys-AuNPs)and studied the effects of chiral-modified AuNPs on osteogenic differentiation and autophagy of human periodontal ligament cells(hPDLCs)and periodontal tissue regeneration.In vitro,more L-Cys-AuNPs than D-Cys-AuNPs tend to internalize in hPDLCs.L-Cys-AuNPs also significantly increased the expression of alkaline phosphatase,collagen type 1,osteocalcin,runt-related transcription factor 2,and microtubule-associated protein light chain 3 II and decreased the expression of sequestosome 1 in hPDLCs compared to the expression levels in the hPDLCs treated by D-Cys-AuNPs.In vivo tests in a rat periodontal-defect model showed that L-Cys-AuNPs had the greatest effect on periodontal-tissue regeneration.The activation of autophagy in L-Cys-AuNP-treated hPDLCs may be responsible for the cell differentiation and tissue regeneration.Therefore,compared to D-Cys-AuNPs,L-Cys-AuNPs show a better performance in cellular internalization,regulation of autophagy,cell osteogenic differentiation,and periodontal tissue regeneration.This demonstrates the immense potential of L-Cys-AuNPs for periodontal regeneration and provides a new insight into chirally modified bioactive nanomaterials.

关 键 词：CHIRALITY Gold nanoparticles PERIODONTAL Tissue regeneration AUTOPHAGY 

分 类 号：R78[医药卫生—口腔医学] R318.08[医药卫生—临床医学]