Hypoxia-mimicking 3D bioglass-nanoclay scaffolds promote endogenous bone regeneration  

作　　者：Xiao Zheng Xiaorong Zhang Yingting Wang Yangxi Liu Yining Pan Yijia Li Man Ji Xueqin Zhao Shengbin Huang Qingqing Yao 

机构地区：[1]Institute of Advanced Materials for Nano-Bio Applications,School of Ophthalmology&Optometry,Eye Hospital,Wenzhou Medical University,270 Xueyuan Xi Road,Wenzhou,Zhejiang,325027,PR China [2]Department of Endodontics,School and Hospital of Stomatology,Wenzhou Medical University,Wenzhou,PR China [3]Institute of Stomatology,School and Hospital of Stomatology,Wenzhou Medical University,Wenzhou,PR China [4]Comprehensive Transplant Center,Feinberg School of Medicine,Northwestern University,USA [5]Department of Surgery,Feinberg School of Medicine,Northwestern University,USA [6]College of Life Science and Medicine,Zhejiang Sci-Tech University,Hangzhou,310018,PR China [7]Department of Prosthodontics,School and Hospital of Stomatology,Wenzhou Medical University,Wenzhou,China

出　　处：《Bioactive Materials》2021年第10期3485-3495,共11页生物活性材料（英文）

基　　金：This work is supported by the Chinese National Natural Science Foundation of China(31600773);Zhejiang Provincial Natural Science Foundation of China(LY18C100002).

摘　　要：Large bone defect repair requires biomaterials that promote angiogenesis and osteogenesis.In present work,a nanoclay(Laponite,XLS)-functionalized 3D bioglass(BG)scaffold with hypoxia mimicking property was prepared by foam replication coupled with UV photopolymerization methods.Our data revealed that the incorporation of XLS can significantly promote the mechanical property of the scaffold and the osteogenic differentiation of human adipose mesenchymal stem cells(ADSCs)compared to the properties of the neat BG scaffold.Desferoxamine,a hypoxia mimicking agent,encourages bone regeneration via activating hypoxia-inducible factor-1 alpha(HIF-1α)-mediated angiogenesis.GelMA-DFO immobilization onto BG-XLS scaffold achieved sustained DFO release and inhibited DFO degradation.Furthermore,in vitro data demonstrated increased HIF-1αand vascular endothelial growth factor(VEGF)expressions on human adipose mesenchymal stem cells(ADSCs).Moreover,BG-XLS/GelMA-DFO scaffolds also significantly promoted the osteogenic differentiation of ADSCs.Most importantly,our in vivo data indicated BG-XLS/GelMA-DFO scaffolds strongly increased bone healing in a critical-sized mouse cranial bone defect model.Therefore,we developed a novel BG-XLS/GelMA-DFO scaffold which can not only induce the expression of VEGF,but also promote osteogenic differentiation of ADSCs to promote endogenous bone regeneration.

关 键 词：3D bioglass scaffold HYPOXIA Angiogenesis OSTEOGENESIS Endogenous bone regeneration 

分 类 号：R318.08[医药卫生—生物医学工程] R68[医药卫生—基础医学]