外源硅处理对草莓果实果胶物质降解的影响  

Effect of exogenous silicon treatment on pectin degradation of strawberry

作　　者：赵文哲 陈修德[1,2,3] 邓文鹏 吴琦杰 杜桂英 和华杰 王伟 周涛 肖伟 李玲[1,2,3] ZHAO Wenzhe;CHEN Xiude;DENG Wenpeng;WU Qijie;Du Guiying;HE Huajie;WANG Wei;ZHOU Tao;XIAO Wei;LI Ling(State Key Laboratory of Crop Biology,Taian,Shandong 271018,China;Shandong Collaborative Innovation Center for Fruit and Vegetable Product!on with High Quality and Efficiency,Taian,Shandong 271018,China;College of Horticulture Scienee and Engineering,Shandong Agricultural University,Taian,Shandong 271018,China;Rizhao City Wulian County Agriculture and Rural Affairs Bureau,Rizhao,Shandong 276800,China;Agricultural Integrated Service Centre of Pingyi Subdistrict Office in Pingyi County,Linyi,Shandong 273300,China)

机构地区：[1]作物生物学国家重点实验室,山东泰安271018 [2]山东果蔬优质高效生产协同创新中心,山东泰安271018 [3]山东农业大学园艺科学与工程学院,山东泰安271018 [4]日照市五莲县农业农村局,山东日照276800 [5]平邑县平邑街道办事处农业综合服务中心,山东临沂273300

出　　处：《植物生理学报》2021年第10期1926-1936,共11页Plant Physiology Journal

基　　金：山东省现代农业产业技术体系果品创新团队项目(SDAIT-06-01)。

摘　　要：果胶是细胞壁初生壁和中胶层的主要成分,影响果实的品质。为探究外源硅处理对草莓果实细胞壁降解影响,试验以‘章姬’草莓(Fragaria×ananassa‘Akihime’)为试材,研究叶面喷硅对草莓绿果期、白果期、转红期及全红期果实细胞壁组分(果胶、原果胶)、细胞壁降解酶[多聚半乳糖醛酸酶(PG)、果胶裂解酶(PL)、纤维素酶(CX)]活性以及PG、PL、果胶甲酯酶(PME)、β-半乳糖苷酶(β-Gal)和内切葡聚糖酶(EG)编码基因表达的影响。结果表明,外源1 g·L^(-1)流体硅处理,可溶性果胶含量,PG、PL、CX活性以及FaPG、FaPL、Faβ-Gal、FaEG基因的表达量在绿果期、白果期、转红期均高于对照;外源5 g·L^(-1)流体硅处理后,可溶性果胶含量,PG、PL、CX活性以及FaPG、FaPL、FaEG基因的表达量在转红期、全红期均低于对照。综上所述,外源5 g·L^(-1)流体硅处理对延缓细胞壁组分代谢的效果最佳,具有较高的应用价值。Pectin is the main component of the primary wall and middle layer of cell wall, which affects the quality of fruit. To explore the effect of exogenous silicon treatment on cell wall degradation of strawberry fruit, experiments were carried with Fragaria × ananassa ‘Akihime’ as test material. The cell wall components(pectin, protopectin), the activities of cell wall degradation enzymes(PG, PL and CX) and the expression of FaPG, FaPL, Faβ-Gal and FaEG genes in green fruit stage, white fruit stage, red turning stage and full red stage of strawberry were studied. The results showed that the content of soluble pectin, the activities of PG, PL and CX, and the expression of FaPG, FaPL, Faβ-Gal and FaEG genes were higher than those of the control in green fruit stage, white fruit stage and red turning stage after exogenous 1 g·L^(-1) fluid silicon treatment;the content of soluble pectin, the activities of PG, PL, CX and the expression of FaPG, FaPL, FaEG genes were lower than those of the control in red turning stage and full red stage after 5 g·L^(-1) fluid silicon treatment. In conclusion, exogenous 5 g·L^(-1) fluid silicon treatment has the best effect on delaying the metabolism of cell wall components and has high application value.

关 键 词：草莓 硅 细胞壁降解 果胶 

分 类 号：S668.4[农业科学—果树学]