EDNRB在前列腺癌中的表达及临床预后意义  

Expression and prognostic significance of EDNRB in prostate cancer

作　　者：李菲 王宇航 徐陈晨 张永静 李伟玉 陆进[2,3] Li Fei;Wang Yuhang;Xu Chenchen;Zhang Yongjing;Li Weiyu;Lu Jin(School of Clinical Medicine,Bengbu Medical College,Bengbu 233030,Anhui,China;Department of Human Anatomy,Bengbu Medical College,Bengbu 233030,Anhui,China;Anhui Key Laboratory of Computational Medicine and Intelligent Health,Bengbu Medical College,Bengbu 233030,Anhui,China)

机构地区：[1]蚌埠医学院临床医学院,安徽蚌埠233030 [2]蚌埠医学院人体解剖学教研室,安徽蚌埠233030 [3]蚌埠医学院数字医学与智慧健康安徽省重点实验室,安徽蚌埠233030

出　　处：《右江民族医学院学报》2021年第5期611-616,共6页Journal of Youjiang Medical University for Nationalities

基　　金：安徽省大学生创新训练项目(S202010367071);教育部产学合作协同育人项目(202101160001)。

摘　　要：目的利用生物信息学方法,筛选前列腺癌预后的关键基因。方法首先,从GEO数据库下载4个前列腺癌表达谱芯片数据集(GSE3325/GSE46602/GSE55945/GSE69223);其次,分别利用GEO2R、DAVID以及STRING数据库分析前列腺癌的差异表达基因(DEGs),并对差异基因进行基因本体论(GO)和京都基因与基因组百科全书(KEGG)通路富集以及蛋白质-蛋白质相互作用关系(PPI);再次,通过Cytoscape软件的Cytohubba插件EPC算法筛选关键基因;最后,利用基因表达谱交互分析(GEPIA)数据库对关键基因进行预后与表达分析,利用人类蛋白图谱(HPA)数据库下载TCGA数据对结果进行验证。结果筛选出103个DEGs,其功能主要富集在血管生成、脂质代谢以及细胞分化等方面,而通路主要富集在Hippo信号上。筛选的10个关键基因中仅EDNRB对前列腺癌患者的预后有显著影响,且其低表达不利于患者预后。结论通过生物学分析筛选前列腺癌关键基因,为其治疗提供新的分子靶点。Objective To screen the key genes for prognosis of prostate cancer by bioinformatics.Methods Firstly,four microarray datasets(GSE3325/GSE46602/GSE55945/GSE69223)of prostate cancer expression profile were downloaded from the GEO database.Secondly,the GEO2R,DAVID and STRING databases were used to analyze differentially expressed genes(DEGs)in prostate cancer.Then the pathway enrichment of gene ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)and the protein-protein interaction(PPI)for DEGs were analyzed.Thirdly,Cytohubba plug-in EPC algorithm of software Cytoscape was used to screen key genes.Finally,the gene expression profile interaction analysis(GEPIA)database was used to analyze the prognosis and expression of key genes,and the results were verified using data downloaded from the Human Protein Atlas(HPA)database.Results A total of 103 DEGs were screened out,and their functions were mainly enriched in angiogenesis,lipid metabolism and cell differentiation,while their pathways were mainly enriched in Hippo signals.Among the 10 key genes screened,only EDNRB had a significant effect on the prognosis of patients with prostate cancer,and its low expression was not conducive to the prognosis of patients.Conclusion Screening key genes of prostate cancer by bioinformatics can provide new molecular targets for its treatment.

关 键 词：前列腺肿瘤 计算生物学 基因 总体生存 预后 

分 类 号：R737.25[医药卫生—肿瘤]