Assessing chromatin condensation for epigenetics with a DNA-targeting sensor by FRET and FLIM techniques  

作　　者：Xinfu Zhang Lu Wang Ning Li Yi Xiao 

机构地区：[1]State Key Laboratory of Fine Chemicals,Dalian University of Technology,Dalian 116024,China

出　　处：《Chinese Chemical Letters》2021年第8期2395-2399,共5页中国化学快报（英文版）

基　　金：supported by the National Natural Science Foundation of China (Nos.21421005,21576040,21776037,21901031 and 22078047);Science and Technology Foundation of Liaoning Province (No.2020-YQ-08);Dalian Science and Technology Innovation Fund (No.2020JJ25CY014);the Fundamental Research Funds for the Central Universities (Nos.DUT18RC(3)027 and DUT20RC(5)024)。

摘　　要：Here we propose a fluorescent sensor,Chroma-V,consisted of a Hoechst ligand(Hoe) to target chromatin DNA and a BODIPY rotor(BDP) to sense the local viscosity that reflects chromatin condensation state.Within Chroma-V,efficient FRET process from Hoe to BDP facilitated a single-excitation ratiometric imaging of nucleus DNA under fluorescence confocal microscope,which utilized the ratio of two channels to enable an intuitive visualization of chromatin condensation state.And fluorescence lifetime imaging(FLIM) based on fluorescent signal from BDP proved to be a more accurate method to quantify the changes of chromatin condensation state under different epigenetic states,including histone acetylation regulated by deacetylase inhibitors,cell apoptosis induced by DNA-bining drugs,and the epithelialmesenchymal transition of HUVEC cells induced by TGF-β.

关 键 词：Ratio imaging Fluorescence lifetime imaging EPIGENETIC CHROMATIN BODIPY rotor Hoechst 

分 类 号：Q343.2[生物学—遗传学] O657.3[理学—分析化学]