Increased cGAS/STING signaling components in patients with Mooren's ulcer  

作　　者：Ya-Ni Zhang Yan-Ling Dong Wen-Pei Hao Xiao-Fei Bai Xia Qi Ting Liu Xiao-Tong Sun Chao Wei Xiao-Lin Qi 

机构地区：[1]Xi'an Children's Hospital,Xi'an 710004,Shaanxi Province,China [2]Shandong Eye Institute,Shandong First Medical University&Shandong Academy of Medical Sciences,Qingdao 266071,Shandong Province,China [3]Qingdao Eye Hospital of Shandong First Medical University,Qingdao 266071,Shandong Province,China [4]State Key Laboratory Cultivation Base,Shandong Provincial Key Laboratory of Ophthalmology,Shandong Eye Institute,Shandong First Medical University&Shandong Academy of Medical Sciences,Qingdao 266071,Shandong Province,China [5]Eye Hospital of Shandong First Medical University,Jinan 250021,Shandong Province,China

出　　处：《International Journal of Ophthalmology(English edition)》2021年第11期1660-1665,共6页国际眼科杂志（英文版）

基　　金：Supported by National Natural Science Foundation of China(No.81900907);the Young and Middle-Aged Scientists Research Awards Fund of Shandong Province(No.ZR2017BH004)。

摘　　要：AIM:To explore the expression of cGAS/STING signaling components in Mooren’s ulcer(MU).METHODS:Samples were obtained from ten MU patients,and eight residual corneal-scleral rings of healthy donor corneas for controls.Human corneal epithelial cells(HCECs)were used to evaluate the effect of cGAS/STING signaling pathway.Immunohistochemistr y(IHC)and Western blot were used to examine the expression of cGAS,STING,and phosphorylated interferon regulatory factor 3(p-IRF3)in MU tissues.The expression of interferon-β(IFN-β)and interferon-stimulated genes(ISGs)was quantified by real-time polymerase chain reaction(PCR)and enzymelinked immunosorbent assay(ELISA).RESULTS:The protein levels of cGAS and STING in MU samples were significantly elevated when compared with the healthy controls by Western blot and IHC.After stimulation with cGAMP,real-time PCR and ELISA showed a dramatic increase of IFN-βand ISGs(containing CXCL10,IFIT1,and IL-6)in HCECs.Moreover,HCECs treated with cGAMP was characterized by increased phosphorylation and more nuclear translocation of IRF3.Meanwhile,increased p-IRF3 was observed in MU samples via IHC and Western blot.CONCLUSION:The pronounced expression of cGAS/STING signaling components in the patients with MU and probably contribute to the onset and development of MU.

关 键 词：cGAS/STING signaling interferon regulatory factor 3 Mooren’s ulcer INTERFERON-Β 

分 类 号：R772.2[医药卫生—眼科]