黑皮油松松针精油纳米乳液的制备及稳定性研究  

Preparation and stability of Pinus tabulaeformis pine needle essential oil nanoemulsion

作　　者：王雪薇 李德海[1] WANG Xuewei;LI Dehai(Forestry College/Key Laboratory of forest food resources utilization of Heilongjiang Province,Northeast Forestry University,Harbin 150040,China)

机构地区：[1]东北林业大学林学院/黑龙江省森林食品资源利用重点实验室,哈尔滨150040

出　　处：《中国农业大学学报》2021年第11期123-132,共10页Journal of China Agricultural University

基　　金：中央高校基本科研业务费专项资金资助(2572019BA04);哈尔滨市科技局创新研究基金项(2017RAQXJ091);黑龙江省自然科学基金面上项目(C2015062)。

摘　　要：为提高黑皮油松松针精油的水溶性和乳化稳定性,本研究利用旋涡混合仪和微射流仪制备纳米乳液,以分层体积和三元相图中的乳化区面积为指标,确定活性剂的种类及配比(Km)等制备纳米乳液的最优条件,并进一步分析了纳米乳液的结构、粒径、多分散项系数(PDI)及稳定性。结果表明:1)黑皮油松松针精油纳米乳液的最佳配方为精油16.14%、复合表面活性剂10.45%(m(无水乙醇)∶m(Tween 80)=4∶1)和蒸馏水73.41%;2)黑皮油松松针精油纳米乳液有淡蓝色微乳光,液滴呈球形且分布均匀,粒径为35nm,PDI为0.165;3)稳定性实验研究表明,在室温条件下储存60d和在pH=7的水相环境中,纳米乳液的平均粒径和PDI无明显变化,表现了较好的储藏稳定性,最佳储藏时间为30d;热稳定性实验表明,松针精油纳米乳液昙点温度为80℃,具有良好的热稳定性;4)抑菌稳定性实验结果显示,与未乳化的精油相比,松针精油纳米乳液具有更强的抑菌活性。本研究制备的黑皮油松松针精油纳米乳液具有较好的水溶性和稳定性,且与精油相比,抑菌能力更强。In order to improve the water solubility and emulsifying stability of Pinus tabulaeformis needle essential oil,the nano emulsion was prepared by the swirling mixer and microfluidic apparatus.By using the volume fraction of the emulsion and the area of the emulsion in three phase diagram,the best conditions for the preparation of nano emulsion were determined and the structure,particle size and multiple fractions of the nano emulsion Km,PDI and stability were analyzed.The results showed that:1)The optimal formula of P.tabulaeformis needle essential oil nanoemulsion was 16.14%of essential oil,10.45%of compound surfactant(4∶1 of absolute alcohol and Tween 80),and 73.41%of distilled water;2)P.tabulaeformis needle essential oil nanoemulsion had light blue microemulsion,and the droplets were spherical and distributed evenly,with a diameter of 35 nm,PDI is 0.165;3)The stability experiment showed that the average particle size and PDI of nanoemulsion had no obvious change under the condition of storing 60 days at room temperature and in pH=7 aqueous phase environment,which showed better storage stability.The best storage time was 30 days.The thermal stability test showed that the temperature of the pine needle essential oil nanoemulsion was 80℃,which had good thermal stability;4)The antibacterial stability experiment results showed that the pine needle essential oil nano emulsion had better antibacterial activity compared with the non-emulsified essential oil.In conclusion,the P.tabulaeformis needle essential oil nanoemulsion prepared in this study had good water solubility and stability,and its bacteriostatic ability was enhanced compared with essential oil.

关 键 词：黑皮油松 精油 纳米乳液 稳定性 

分 类 号：TS202.3[轻工技术与工程—食品科学]