S100A7 Co-localization and Up-regulation of Filaggrin in Human Sinonasal Epithelial Cells  

作　　者：Masahiro Nakamura Kazusaku Kamiya Atsushi Furuhata Katsuhisa Ikeda Frangois Niyonsaba 

机构地区：[1]Department of Otorhinolaryngology,Juntendo University School of Medicine,Tokyo 113-8421,Japan [2]Atopy(Allergy)Research Center,Junlendo University School of Medicine,Tokyol 13-8421,Japan [3]Biomedical Research Center,Graduate School of Medicine,Juntendo University,Tokyo 113-8421,Japan [4]Faculty of International Liberal Arts,Laboratory of Morphology and Image Analysis,Graduate School of Medicine,Juntendo University,Tokyo 113-8421,Japan

出　　处：《Current Medical Science》2021年第5期863-868,共6页当代医学科学（英文）

摘　　要：Objective:Filaggrin(FLG)is a protein expressed in the epidermis and involved in the maintenance of the epidermal barrier.However,the expression and localization of FLG in the upper airway remain controversial.The present study aimed to determine the significance of FLG and the effect of S100A7 on FLG expression in the upper respiratory mucosa.Methods:Human nasal epithelial cells(HNECs)were cultured and examined for FLG expression and S100A7 effects by real-time polymerase chain reaction and Western blotting.The localization and distribution of FLG were assessed using sinonasal mucosa.Results:A significant expression of FLG was detected at the mRNA and protein levels in HNECs.A moderate FLG immunoreactivity was observed in the epithelial cells,but no staining was seen in epithelial goblet cells.S100A7 increased the FLG mRNA level in HNECs in a dose-dependent manner and also up-regulated the FLG protein in a dose-dependent manner.Conclusion:This study significantly contributes to a better understanding of the role of FLG in the pathogenesis of airway inflammation from the viewpoint of the epithelial barrier function.FLG-related events in response to S100A7 protein may represent novel therapeutic targets for the treatment of upper airway inflammation.

关 键 词：nasal epithelial cell FILAGGRIN S100A7 KERATINOCYTE 

分 类 号：R73[医药卫生—肿瘤]