鱼皮胶原蛋白中透明质酸酶抑制肽的制备工艺优化  

Optimization for preparation of collagen peptide with hyaluronidase inhibition activity by response surface method

作　　者：韩秋煜 石崎松一郎 包斌[1,3] HAN Qiu-yu;ISHIZAKI Shioichiro;BAO Bin(College of Food Science and Technology,Shanghai Ocean University,Shanghai 201306,China;Graduate School of Marine Science and Technology,Tokyo University of Marine Science and Technology,Tokyo 1080075,Japan;National Experimental Teaching Demonstration Center for Food Science and Engineering〔Shanghai Ocean University〕,Shanghai 201306,China)

机构地区：[1]上海海洋大学食品学院,上海201306 [2]东京海洋大学海洋技术研究科,日本东京1080075 [3]食品科学与工程国家级实验教学示范中心〔上海海洋大学〕,上海201306

出　　处：《食品与机械》2021年第11期142-147,共6页Food and Machinery

基　　金：上海市创新行动计划(编号:19440741200);国家自然科学基金(编号:82173731)。

摘　　要：目的:高效提取胶原蛋白中的透明质酸酶抑制肽。方法:以剑鱼(Xiphias gladius)鱼皮为原料,使用胃蛋白酶提取酶溶性胶原蛋白并使用盐析法进行纯化,经聚丙烯酰胺凝胶电泳鉴定胶原蛋白种类;通过单因素和响应面法优化透明质酸酶抑制肽的提取工艺。结果:使用胃蛋白酶提取的胶原蛋白为Ⅰ型胶原,透明质酸酶抑制性胶原蛋白肽的优化提取条件为复合酶比(m_(胶原蛋白酶)∶m_(蛋白酶))8∶2,酶解温度32℃,酶解时间6 h,加酶量6%。在此条件下胶原蛋白肽的透明质酸酶抑制率为42.35%,响应面模型拟合性好,透明质酸酶抑制率的预测值与实际值无显著差异(P>0.05)。结论:该工艺可应用于对剑鱼鱼皮中具有透明质酸酶抑制性的胶原蛋白肽的提取。Objective:The extraction process of collagen peptides from the skin of swordfish(Xiphias gladius)was optimized in order to efficiently obtain collagen peptides with hyaluronidase inhibition activity by single factor and response surface method.Methods:The response surface method was used to optimize the preparation of collagen peptide with hyaluronidase inhibition activity.In this paper,the enzyme-soluble collagen was extracted from swordfish skin by pepsin,and then the salting-out method was carried out to purify the collagen initially.The specie of collagen was identified by polyacrylamide gel chromatography with 7.5%separation gel.After the basic test carried out by single-factor method according to the degree of hydrolysis and hyaluronidase inhibition rate,the hyaluronidase inhibition rate was selected as the response value for the optimization of hydrolysis conditions of collagen peptide derived from the skin of swordfish via Box-Behnken response surface experimental method.Results:The results showed that the pepsin-soluble collagen was identified as the type Ⅰ collagen.The optimized hydrolysis conditions for the type Ⅰ collagen peptide with hyaluronidase inhibition activity were:6 h of hydrolysis time,8∶2 of complex enzyme ratio,32℃of hydrolysis temperature and 6%of enzyme mixture addition.The hyaluronidase inhibition rate of the type Ⅰ collagen peptide under these conditions was 42.35%.According to the statistical analysis,the response surface model fitted well and there was no significant difference between the predicted and actual values of hyaluronidase inhibition rate(P>0.05).Conclusion:Therefore,this hydrolysis process could be applied to the production of type Ⅰ collagen peptide with hyaluronidase inhibition activity derived from the skin of swordfish.

关 键 词：胶原蛋白肽 透明质酸 剑鱼 Ⅰ型胶原 

分 类 号：TS254.9[轻工技术与工程—水产品加工及贮藏工程]