基于RNA-Seq数据分析油酸与胰岛素诱导延边黄牛前脂肪细胞分化的差异表达基因  

Analysis of differentially expressed genes in oleic acid and insulin induced preadipocyte differentiation in Yanbian yellow cattle based on RNA-Seq data

作　　者：郭盼盼 金鑫 孙建富 李强 李香子 严昌国 尹云厚[2] GUO Panpan;JIN Xin;SUN Jianfu;LI Qiang;LI Xiangzi;YAN Changguo;YIN Yunhou(Collaborative Innovation Center of Beef Cattle Science and Industrial Technology,Yanbian University,Yanji,Jilin 133000,China;Guizhou Minzu University,Guiyang 550025,China;Yanbian Hongchao Animal Husbandry Group Co.,Ltd,Yanji,Jilin 133000,China)

机构地区：[1]延边大学肉牛科学与产业技术协同创新中心,吉林延吉133000 [2]贵州民族大学,贵州贵阳550025 [3]延边弘朝畜牧集团有限公司,吉林延吉133000

出　　处：《中国兽医学报》2021年第11期2167-2175,2181,共10页Chinese Journal of Veterinary Science

基　　金：高等学校学科创新引智计划资助项目(D20034);国家重点研发计划资助项目(2018YFD0501702);贵州省科技计划资助项目(黔科合基础[2018]1073)。

摘　　要：本试验旨在通过RNA-Seq技术来探究油酸和胰岛素在延边黄牛前脂肪细胞分化途径中可能的调控机制。外源性添加油酸和胰岛素对延边黄牛前脂肪细胞进行体外诱导分化,处理96 h后进行转录组测序。试验分为对照组(CON,5%FBS)、胰岛素处理组(I,5%FBS+10 mg/L胰岛素)、油酸处理组(O,5%FBS+100μmol/L油酸)以及胰岛素+油酸处理组(IO,5%FBS+10 mg/L胰岛素+100μmol/L油酸)。各试验组差异表达基因(differentially expressed genes,DEGs)分析结果显示:I组、O组和IO组相比于CON组,分别发现1488,1773和1974个DEGs,且各试验组共有435个相同的DEGs;GO分析表明大多数DEGs与细胞成分组织或生物发生、多细胞有机体过程、生物过程调控、生物调节、代谢过程及细胞过程等生物途径有关;在分子功能上,主要是转运活性、转录调节活性、分子功能调节及催化活性等;KEGG富集分析结果显示:O组和IO组的DEGs积极调控脂肪细胞代谢,包括脂肪酸代谢、PPARγ信号通路、AMPK信号通路、胆固醇代谢;I组的DEGs参与氨基酸生物合成信号通路、TGF-β信号通路以及Wnt信号通路等。综合分析结果表明,胰岛素的添加至少部分地加强了油酸本身对前体脂肪细胞分化的促进作用。本试验结果为进一步研究油酸和胰岛素在调节脂肪生成中的功能和分子机制提供了依据。The purpose of this study was to explore the regulatory mechanism of oleic acid and insulin in the differentiation pathway of preadipocytes in Yanbian yellow cattle by RNA-Seq technique.Exogenous oleic acid and insulin were added to induce and differentiate the preadipocytes of Yanbian yellow cattle in vitro,and transcriptome sequencing was performed after 96 h treatment.The experiments were divided into the control group(CON,5%FBS),the insulin treatment group(I,5%FBS+10 mg/L insulin),oleic acid treatment group(O,5%FBS+100μmol/L oleic acid)and the insulin+oleic acid treatment group(IO,5%FBS+10 mg/L insulin+100μmol/L oleic acid).The analysis of differentially expressed genes(DEGs)in different groups showed that compared with CON group,1488,1773 and 1974 DEGs were found in group I,group O and group IO.There were 435 DEGs in each treatment group.GO analysis showed that most DEGs were related to biological pathways such as cellular component organization or biogenesis,multicellular organism process,biological process regulation,biological regulation,metabolic process and cellular process.In terms of molecular function,it mainly involved in transport activity,transcriptional regulation activity,molecular function regulation and catalytic activity,etc.KEGG enrichment analysis showed that DEGs in the O and IO groups actively regulated adipocyte metabolism,including fatty acid metabolism,PPAR signaling pathway,AMPK signaling pathway,and cholesterol metabolism.DEGs in group I were involved in amino acid biosynthesis signaling pathway,TGF signaling pathway and Wnt signaling pathway.The comprehensive analysis results showed that the addition of insulin at least partially enhanced the promoting effect of oleic acid on the differentiation of precursor adipocytes.The results of this study provide a basis for further research on the function and molecular mechanism of oleic acid and insulin in regulating fat production.

关 键 词：延边黄牛 前脂肪细胞 RNA-SEQ 胰岛素 油酸 

分 类 号：S852.2[农业科学—基础兽医学] S823.81[农业科学—兽医学]