Elemene Emulsion Injection Administration Reduces Neuropathic Pain by Inhibiting Astrocytic NDRG2 Expression within Spinal Dorsal Horn  

作　　者：MA Li-tian BAI Yang LI Jie QIAO Yu LIU Yang ZHENG Jin 

机构地区：[1]Department of Traditional Chinese Medicine,Tangdu Hospital,the Fourth Military Medical University,Xi'an(710038),China [2]Department of Gastroenterology,Tangdu Hospital,Air Force Medical University,Xi'an(710032),China [3]Department of Anatomy,Histology and Embryology&K.K.Leung Brain Research Centre,the Fourth Military Medical University,Xi'an(710032),China [4]Department of Neurosurgery,General Hospital of Northern Theater Command(General Hospital of Shenyang Military Command),Shenyang(110016),China [5]Department of Endocrinology,Xijing Hospital,the Fourth Military Medical University,Xi'an(710038),China [6]Laser Medical Center,Hainan Hospital,People's Liberation Army General Hospital,Sanya(572013),Hainan Province,China [7]Health Center of 31658 of the People's Liberation Army,Xining(810000),China

出　　处：《Chinese Journal of Integrative Medicine》2021年第12期912-918,共7页中国结合医学杂志（英文版）

基　　金：the Science and Technology Co-ordination Innovation Project Plan of Shaanxi Province(No.2016KTCL03-16)。

摘　　要：Objective To investigate the mechanisms underlying elemene-induced analgesia in rats with spared nerve injury(SNI).Methods Sixty-five rats were equally divided into 5 groups using a random number table:naive group,sham group,SNI group,SNI+elemene(40 mg·kg−1·d−1)group and naive+elemene(40 mg·kg−1·d−1)group.An SNI rat model was established and the intervention were given respectively for 14 consecutive days.Von Frey filament tests and elevated plus-maze(EPM)tests were used to evaluate the effect of elemene on the mechanical threshold and anxiety,respectively.Immunoblotting and immunostaining were used to measure the expression of glial fibrillary acidic protein(GFAP)and NMYC downstream-regulated gene 2(NDRG2)within the lumbar spinal dorsal horn(SDH).Results The SNI rat model exhibited a significant decrease in paw withdrawal threshold and exploratory behaviour in the EPM(P<0.05).Consecutive administration of elemene alleviated SNI-induced mechanical allodynia and anxiety in rats(P<0.05).Immunohistochemical data showed that elemene decreased SNI-induced upregulation of NDRG2 within the SDH(P<0.05).Double immunofluorescent staining data further showed that elemene decreased SNI-induced upregulation of the number of GFAP immunoreactive(-ir),NDRG-ir,and GFAP/NDRG2 double-labelled cells within the SDH(P<0.05).Immunoblotting data showed that elemene decreased SNI-induced upregulation of GFAP and NDRG2 within the SDH(P<0.05).Conclusion Elemene possibly alleviated neuropathic pain by downregulating the expression of NDRG2 in spinal astrocytes in a rat model of SNI.

关 键 词：elemene emulsion injection ASTROCYTE NMYC downstream-regulated gene 2 

分 类 号：R741.05[医药卫生—神经病学与精神病学]