ALDH2、ADH1B基因多态性在不同民族酒依赖者中的差异及其与饮酒行为的关系  

The comparative analyses of ALDH2 and ADH1B gene polymorphism in alcoholism patients among different ethnic and association with drinking behavior

作　　者：徐斌[1] 罗晓[1] 张红 胡红星[1] XU Bin;LUO Xiao;ZHANG Hong;HU Hongxing(Psychological Medicine Center of the First Affiliated Hospital of Xinjiang Medical University,Urumqi,830054;Graduate School of Xinjiang Medical University,Urumqi,830054)

机构地区：[1]新疆医科大学第一附属医院心理医学中心,乌鲁木齐830054 [2]新疆医科大学研究生院,乌鲁木齐830054

出　　处：《中国药物依赖性杂志》2021年第5期358-364,共7页Chinese Journal of Drug Dependence

基　　金：国家自然科学基金项目(81660231)。

摘　　要：目的:酒精代谢酶基因SNP多态性在汉族与维吾尔族酒依赖者中的差异及与个体饮酒行为的关系。方法:所有受试者完成一般情况调查表,抽取静脉血提取DNA,检测乙醛脱氢酶2(ALDH2 rs671)、乙醇脱氢酶1B(ADH1B rs1229984)、乙醇脱氢酶1C(ADH1C rs141973904)多态性。利用效用程序计算遗传连锁分析的Hardy-Weinberg平衡(HWE)。采用Pearson′s卡方检验并计算优势比OR值,单因素ANOVA方差分析的方法分析不同基因型SNP位点与饮酒的相关性。结果:汉族与维吾尔族酒依赖者及对照组中均以ALDH2*2(χ^(2)=1.836,P=0.175)及ADH1C*1(χ^(2)=1.154,P=0.283)等位基因型为主,差异无统计学意义;汉族酒依赖者及对照组中ADH1B*2基因型者均多于维吾尔族(χ^(2)=17.838,P=0.000)。汉族与维吾尔族受试者中ALDH2等位基因位点A与酒依赖的风险相关(P_(汉)=0.00,OR=7.111,95%CI[2.928~17.30];P_(维)=0.015,OR=5.47,95%CI[1.190~25.02])。两个民族中均显示ADH1B等位基因位点T是酒依赖的保护性因素。两个民族酒依赖者中只有ADH1B*2的不同等位基因型与饮酒量有关系。rs1229984 TT型者每日最大饮酒量(F_(汉)=4.86,P=0.01;F_(维)=21.52,P<0.01)、周饮酒量(F_(汉)=4.51,P=0.01;F_(维)=14.12,P<0.01)均低于CC或CT型者。结论:汉族与维吾尔族受试者中无论酒依赖者还是对照者,均以ALDH2*2及ADH1C*1等位基因型者为主,ADH1B*2不同等位基因型与个体饮酒量有关系,且维吾尔族受试者无论酒依赖者还是对照组ADH1B*2型均少于汉族对应人群。ALDH2*2及ADH1B*2均为酒依赖的保护性因素。Objective:The difference of gene polymorphism in alcohol metabolizing enzyme between Han and Uygur alcohol dependence patients and their relationship with individual drinking behavior.Methods:After completing the general demographics questionnaire,every subject was extracted 5 ml venous blood for DNA extraction,and polymorphism of Acetaldehyde dehydrogenase 2(ALDH2 rs671),ethanol dehydrogenase(ADH1B rs1229984 and ADH1C rs141973904)was detected.Hardy-weinberg Equilibrium(HWE)for genetic linkage analysis is calculated by utility program.Pearson′s Chi-square test was used to calculate the odds ratio(OR),and the correlation between SNP loci of different genotypes and alcohol consumption was analyzed by one-way ANOVA.Results:There was no statistically significant difference in ALDH2*2 and ADH1C*1 alleles between Han and Uygur alcoholism patients and the control group,and the two alleles were predominant in all subjects(ALDH2:χ^(2)=1.836,P=0.175;ADH1 C:χ^(2)=1.154,P=0.283).The ADH1B*2 genotypes in Han alcoholism patients and control group were more than uygur subjects(χ^(2)=17.838,P=0.000).ALDH2 allele A was associated with the risk of alcohol dependence in Han and Uygur subjects(Han:P=0.00,OR=7.111,95%CI[2.928-17.30];Uyghur:P=0.015,OR=5.47,95%CI[1.190-25.02]).The allele T of ADH1B was shown to be a protective factor of alcohol dependence in both groups.Only ADH1B*2 polymorphisms were associated with alcohol consumption in both ethnic drinkers.The patients with rs1229984 TT type had lower daily(Han:F=4.86,P=0.01;Uyghur:F=21.52,P<0.01)and weekly(Han:F=4.51,P=0.01;Uyghur:F=14.12,P<0.01)alcohol consumption than those with CC or CT type.Conclusion:ALDH2*2 and ADH1C*1 alleles were dominant in both Han and Uygur subjects,and ADH1B*2 alleles were correlated with the amount of alcohol consume.ADH1B*2 alleles were more rich both in Urgur patients and controls than in those of Han subjects.ALDH2*2 and ADH1 B*2 were protective factors for alcohol dependence in both Han and Uygur population.

关 键 词：酒依赖 酒精代谢酶 民族 等位基因型 饮酒量 

分 类 号：R749[医药卫生—神经病学与精神病学]