罗勒挥发油包合物制备及抑菌活性研究  被引量:3

Preparation and research on antibacterial activity of inclusion compound of basil volatile oil

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作  者:毛见智 刘晓会[1] MAO Jianzhi;LIU Xiaohui(School of Chemistry and Chemical Engineering,Shanghai University of Engineering Science,Shanghai 201620,China)

机构地区:[1]上海工程技术大学化学化工学院,上海201620

出  处:《上海工程技术大学学报》2021年第2期118-122,共5页Journal of Shanghai University of Engineering Science

摘  要:水蒸气蒸馏法提取挥发油后,用羟丙基-β-环糊精制备包合物,用紫外分光光度计测定其含油量.通过显微镜法、薄层色谱(TLC)法、红外光谱(IR)法对其结构进行表征.结果显示,包合物收率为52.23%,包合率为2.82%,含油率为1.02%.采用纸片法和二倍稀释法测定包合物对大肠杆菌、金黄色葡萄球菌和白色念珠菌的抑菌圈和最小抑菌浓度(MIC),结果显示抑菌圈直径分别为1.74、0.82和1.12 cm,最小抑菌浓度分别为3.79、8.58和3.79 mg/mL.After the volatile oil was extracted by steam distillation,hydroxypropyl-β-cyclodextrin was used to refine the inclusion compound,and the oil content was determined by ultraviolet spectrophotometer.Characterization was accomplished by microscope,thin-layer chromatography(TLC)and infrared spectroscopy(IR).The results show that the yield of inclusion compound is 52.23%,the inclusion rate is 2.82%,and the oil content is 1.02%.The inhibition zone and minimum inhibitory concentration(MIC)of the inclusion complex against Escherichia coli,Staphylococcus aureus and Candida albicans were determined by disk method and two-fold dilution method.The results show that the inhibition zone are 1.74,0.82 and 1.12 cm respectively,and the minimum inhibitory concentration are 3.79,8.58 and 3.79 mg/mL respectively.

关 键 词:羟丙基-Β-环糊精 罗勒精油 包合物 抑菌活性 

分 类 号:R284.2[医药卫生—中药学]

 

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