Interaction of IL-22/IL-22R1 promotes cell proliferation and suppresses apoptosis of colorectal cancer via phosphorylation of STAT3  

作　　者：XIAONING QIN LIQING YUAN HONGXUN RUAN LIN LIN 

机构地区：[1]The Third General Surgery Department,The Second Affiliated Hospital of Hebei Medical University [2]The Second Gynecology Department,The Second Affiliated Hospital of Hebei Medical University

出　　处：《BIOCELL》2019年第2期89-98,共10页生物细胞（英文）

摘　　要：Interleukin-22(IL-22)is a member of IL-10 cytokine family which is expressed in activated T cells predominantly and in activated natural killer cells at lower levels.Previous studies have demonstrated the link between elevated levels of IL-22 and disease severity of psoriasis,Crohn’s disease,rheumatoid arthritis and interstitial lung diseases.However,the function of IL-22 in the development and progression of colorectal cancer(CRC)remains elusive.In this study,we first evaluated the IL-22/IL-22R1 level in CRC patients,and found that tumor tissues have more active expression of IL-22 and IL-22R1 than normal tissues,presenting correlation with the degree of differentiation of tumor tissues.Subsequently,caspase and cell viability assays were performed on SW-480 cell line,which expresses high level of IL-22R1,to examine if the supplementation of IL-22 has an impact on apoptosis and proliferation.In comparison with treatment of 5-FU,supplementation of IL-22 promoted cell proliferation and ameliorated apoptosis.To unveil signal transduction upon activation of IL-22R,we examined the phosphorylation of STAT3 in SW-480 cell line following supplementation of IL-22.The treatment of IL-22 also increased the level of p-Akt,an essential component in PI3K/Akt pathway.Although the link between STAT3 phosphorylation and PI3K/Akt activation remains to be explored,our study revealed the mechanism underlying the effects of IL-22R activation on apoptosis as well as tumor differentiation,indicating the prognostic value of IL-22/IL-22R.

关 键 词：INTERLEUKIN-22 Colorectal cancer PI3K/Akt signaling pathway STAT3 

分 类 号：R73[医药卫生—肿瘤]