降解1,3-二甲基-2-咪唑烷酮细菌的分离及功能评价  

Isolation of 1,3-dimethyl-2-imidazolidinone degrading bacteria and evaluation of its function

作　　者：张树林 韩绍良 张海亮 郭雪娜[3] 程艳飞[3] 何秀萍[3,4] ZHANG Shulin;HAN Shaoliang;ZHANG Hailiang;GUO Xuena;CHENG Yanfei;HE Xiuping(Cangzhou Kangzhuang Bio-Fermentation Products Company Limited,Cangzhou,Hebei 061001,China;State Key Laboratory of Direct-Fed Microbial Engineering,Beijing Dabeinong Science and Technology Group Company Limited,Beijing 100192,China;Key Laboratory of Microbial Physiological and Metabolic Engineering,Institute of Microbiology,Chinese Academy of Sciences,Beijing 100101,China;College of Life Sciences,University of Chinese Academy of Sciences,Beijing 100049,China)

机构地区：[1]沧州市康壮生物发酵制品有限公司,河北沧州061001 [2]北京大北农科技集团股份有限公司饲用微生物工程国家重点实验室,北京100192 [3]中国科学院微生物研究所微生物生理与代谢工程重点实验室,北京100101 [4]中国科学院大学生命科学学院,北京100049

出　　处：《微生物学通报》2021年第11期4046-4053,共8页Microbiology China

基　　金：沧州市科技计划项目(181004002D,142202001D)。

摘　　要：【背景】1,3-二甲基-2-咪唑烷酮(1,3-Dimethyl-2-Imidazolidinone,DMI)作为一种强极性非质子溶剂,在生产和应用过程的环境中有稳定残留问题,存在安全隐患。【目的】分离筛选具有降解DMI能力的微生物菌株,为清除环境中残留的DMI提供优良的微生物菌种资源。【方法】从DMI生产区域土壤采集样品分离DMI抗性微生物,采用形态学及分子生物学鉴定确定其分类地位,并对DMI降解能力进行测定。【结果】分离到最高能够耐受5%(体积分数)DMI的微生物菌株,形态学及分子生物学鉴定初步表明获得的菌株DT-1和DT-2为贝莱斯芽孢杆菌(Bacillus velezensis);全细胞及细胞提取液均具有降解DMI的能力;其中菌株DT-1及其细胞提取液对1%(体积分数)DMI的降解率分别达到48%和68%。【结论】从DMI生产区域土壤中分离到具有DMI降解能力的芽孢杆菌,不但可为DMI污染的微生物治理提供优良微生物资源,而且扩展了人们对芽孢杆菌生物学功能的认识。[Background]1,3-Dimethyl-2-imidazolidinone(DMI)is a nonproton solvent with strong polarity,which remains steadily in waste water from production and application processes and causes potential hazard to people’s health.[Objective]Acquisition of DMI degrading microbial strains.[Methods]Isolation and screen of DMI degrading microbial strains from the soil of manufacture plant by DMI tolerance assay.The strains were identified by morphological features and 16 S rRNA gene sequence analysis.[Results]Two putative Bacillus velezensis strains tolerant to 5%(V/V)DMI were isolated from the soil samples,which were designated as DT-1 and DT-2 respectively.Both the whole cells and the cell-free extracts could reduce the DMI levels in the reaction systems.The degrading rates of whole cells and cell-free extract of strain DT-1 reached 48%and 68%respectively at 1%(V/V)DMI concentration.[Conclusion]DMI degrading Bacillus strains were obtained,which provided a basis for microbial degradation of DMI in the polluted environments.Moreover,the results in this study expand our knowledge of the biological function of Bacillus.

关 键 词：1 3-二甲基-2-咪唑烷酮 微生物降解 贝莱斯芽孢杆菌 

分 类 号：X172[环境科学与工程—环境科学]