原儿茶醛对福氏志贺菌的抑制作用  

Inhibitory Effect of Protocatechuicaldehyde against Shigella flexneri and Its Mechanism

作　　者：宋璐忆 李佳辉 王硕 郭嘉璐 邓海潮 石超[1] Song Luyi;Li Jiahui;Wang Shuo;Guo Jialu;Deng Haichao;Shi Chao(College of Food Science and Engineering,Northwest A&F University,Yangling 712100,Shaanxi)

机构地区：[1]西北农林科技大学食品科学与工程学院,陕西杨凌712100

出　　处：《中国食品学报》2021年第11期62-70,共9页Journal of Chinese Institute Of Food Science and Technology

基　　金：国家自然科学基金青年科学基金项目(31801659);陕西省博士后科研项目(2018BSHEDZZ150)。

摘　　要：为寻求安全、有效的福氏志贺菌控制方法,探究原儿茶醛对福氏志贺菌的抑制作用。测定原儿茶醛对福氏志贺菌的最小抑菌浓度、最小杀菌浓度以及其对菌体细胞形态、胞内ATP浓度、膜电位、菌体蛋白质合成和破坏及生长曲线的影响,结果表明:原儿茶醛对福氏志贺菌的最小抑菌浓度为0.5 mg/mL,最小杀菌浓度为4.0 mg/mL。场发射扫描电镜观测到原儿茶醛使福氏志贺菌形态受损,菌体皱缩干瘪;原儿茶醛影响福氏志贺菌细胞膜的通透性,表现为质量浓度为2×MIC和4×MIC的原儿茶醛使细菌胞内ATP浓度显著降低,引起细菌膜电位超极化,破坏菌体蛋白质并抑制细胞蛋白质合成;原儿茶醛延长了福氏志贺菌的生长迟滞期,减小其最大生长速率。结论:原儿茶醛对福氏志贺菌具有良好的抑制作用,其有作为天然、安全的抑菌物质应用于食品工业中的潜力。In order to find a safe and effective control method of Shigella flexneri,the study investigated the inhibitory effect of protocatechuicaldehyde(PC)against S.flexneri and its possible mechanism.The minimum inhibitory concentration(MIC),the minimum bactericidal concentration(MBC)of PC against S.flexneri were determined.Besides,cell morphology potential,the changes of intracellular ATP concentration,membrane potential,the effects on synthesis and destruction of protein as well as the growth curve were also measured to investigate the possible antimicrobial mechanism of PC against S.flexneri.The results showed that the MIC of PC against S.flexneri was 0.5 mg/mL and the MBC was 4.0 mg/mL.Field emission scanning electron microscopy showed that the morphology of S.flexneri was damaged,while PC treated cells were wrinkled and shriveled.In addition,PC affected the cell membrane permeability of S.flexneri,with mass concentration of 2×MIC and 4×MIC reduced the intracellular ATP concentration,cells treated with PC showed hyperpolarized cell membranes.It also destroyed cell proteins and inhibits cell protein synthesis.Moreover,after treatment with PC,the growth retardation period of S.flexneri was prolonged and the specific growth rate was reduced.In conclusion,PC has good antimicrobial activity against S.flexneri,it has the potential to be used in food industry as a natural and safe bacteriostatic substance.

关 键 词：原儿茶醛 福氏志贺菌 细胞膜 抑菌作用 

分 类 号：TS201.3[轻工技术与工程—食品科学]