基于低共熔溶剂分离黑种草子油中抗氧化活性物质  

Separation of antioxidant activity components from black cumin seed oil by deep eutectic solvents

作　　者：伏琳玥 刘学 FU Linyue;LIU Xue(School of Chemical&Material Engineering,Jiangnan University,Wuxi 214122,Jiangsu,China)

机构地区：[1]江南大学化学与材料工程学院,江苏无锡214122

出　　处：《中国油脂》2021年第11期44-49,共6页China Oils and Fats

摘　　要：制备了13种低共熔溶剂(DES),用于分离黑种草子油中抗氧化活性物质。以DPPH自由基清除能力为指标,比较了不同理化性质的DES的萃取效率,通过快速制备色谱法对最优DPPH自由基清除能力的DES萃取物中抗氧化活性物质进一步分离,以UPLC-Q-TOF MS对抗氧化活性高的流分进行成分分析。结果表明:以DES-9(甜菜碱-乙酰丙酸-水,摩尔比1∶3∶2)为萃取溶剂,所得萃取物的抗氧化活性最高,在相同生药质量浓度(3.78 mg/mL)下,其对DPPH自由基的清除率约是传统溶剂(70%甲醇)萃取物的2.6倍。经初步鉴定,DES-9萃取物中抗氧化活性成分主要为3,5-二甲氧基-4-甲基苯基戊酸、苏葛三醇、肉桂酸、Odoratol、2-O-2-苯乙基-β-D-吡喃葡萄糖苷、百里醌、藏花酸、鼠尾草酸。Thirteen deep eutectic solvents(DES)were prepared for separation of antioxidant activity components from black cumin seed oil.The extraction efficiency of different DES was studied based on the DPPH free radical scavenging capability of DES extracts.The antioxidant components in the DES extract with the highest DPPH free radical scavenging capability were further separated by rapid preparation chromatography,and characterized by UPLC-Q-TOF MS.The results showed that the DES-9(betaine-levulinic acid-water,molar ratio 1∶3∶2)extract had the highest antioxidant activity,and the DPPH free radical scavenging rate of DES-9 extract was 2.6 times that of traditional solvent(70%methanol)extract at the same raw material concentration(3.78 mg/mL).The antioxidant components in the DES-9 extract were mainly 5-(3,5-dimethoxy-4-methylphenyl)pentanoic acid,sugetriol,cinnamic acid,odoratol,2-O-2-phenylethyl-β-D-glucopyranoside,thymoquinone,crocetin and carnosic acid.

关 键 词：低共熔溶剂 黑种草子油 抗氧化活性物质 

分 类 号：TS225[轻工技术与工程—粮食、油脂及植物蛋白工程] R284.2[轻工技术与工程—食品科学与工程]