高脂饮食对幽门螺杆菌感染胃黏膜损伤小鼠胃肠激素的影响  

Influence of high-fat diet on Helicobacter pylori infection-induced gastric mucosal injury and gastrointestinal hormones of mice

作　　者：窦广仙 贾惠娟[1] 王萍 邵立风 郑国福 DOU Guang-xian;JIA Hui-juan;WANG Ping;SHAO Li-feng;ZHENG Guo-fu(Tianjin Fifth Central Hospital,Tianjin 300450,China)

机构地区：[1]天津市第五中心医院中心内镜,天津300450

出　　处：《中华医院感染学杂志》2021年第22期3391-3396,共6页Chinese Journal of Nosocomiology

基　　金：天津市科技支撑计划基金资助项目(2018DK156)。

摘　　要：目的探讨高脂饮食对幽门螺杆菌(Hp)感染胃黏膜损伤小鼠胃肠激素的影响。方法将SPF级C57BL/6小鼠分为对照组、高脂饮食组、Hp感染组、高脂饮食+Hp感染组,每组各15只,苏木精-伊红染色法(HE)观察各组小鼠胃黏膜损伤情况和胃黏膜损伤评分,酶联免疫吸附法(ELISA)检测各组小鼠胃组织、血浆胃肠激素水平和胃组织炎性因子水平,蛋白免疫印迹法(Western blot)和逆转录聚合酶链式反应(RT-PCR)检测Wnt/β-catenin信号通路相关蛋白、基因表达水平。结果 HE结果显示高脂饮食组、Hp感染组胃黏膜损伤评分高于对照组(P<0.05),高脂饮食+Hp感染组胃黏膜损伤评分高于高脂饮食组、Hp感染组(P<0.05)。高脂饮食组、Hp感染组胃组织和血浆5-羟色胺(5-HT)、胃泌素(Gas)、胃动素(MTL)水平均高于对照组(P<0.05),而生长抑素(SS)和胃饥饿素(Grelin)水平低于对照组(P<0.05);高脂饮食+Hp感染组胃组织和血浆5-HT、Gas、MTL水平均高于高脂饮食组、Hp感染组(P<0.05),而SS、Grelin水平低于高脂饮食组、Hp感染组(P<0.05)。高脂饮食组、Hp感染组的胃组织肿瘤坏死因子-ɑ(TNF-ɑ)、白细胞介素(IL)-6、IL-1β水平高于对照组(P<0.05),而IL-10低于对照组(P<0.05);高脂饮食+Hp感染组胃组织TNF-ɑ、IL-6、IL-1β水平高于高脂饮食组、Hp感染组(P<0.05),而IL-10含量低于高脂饮食组、Hp感染组(P<0.05)。高脂饮食组、Hp感染组胃组织中Wnt3a和β-catenin蛋白和mRNA水平高于对照组(P<0.05),高脂饮食+Hp感染组胃组织中Wnt3a和β-catenin蛋白和mRNA水平均高于高脂饮食组、Hp感染组(P<0.05)。结论高脂饮食会加重Hp感染诱导的胃黏膜损伤,还能加重胃组织炎症反应和胃肠激素紊乱以及影响Wnt/β-catenin信号通路。OBJECTIVE To explore the influence of high-fat diet on Helicobacter pylori infection-induced gastric mucosal injury and gastrointestinal hormones of mice. METHODS The SPF-level C57 BL/6 j mice were divided into the control group, the high-fat diet group, the Hp infection group and the high-fat diet + Hp infection group, with 15 mice in each group. The gastric mucosal lesions were observed by hematoxylin-eosin(HE) staining so as to obtain the scores of gastric mucosal lesions. The levels of gastrointestinal hormones in gastric tissues and plasma and inflammatory factors in gastric tissues were detected by enzyme-linked immunosorbent assay(ELISA), and the expression levels of Wnt/β-catenin signal pathway-related proteins and genes were detected by means of Western blot and reverse transcription-polymerase chain reaction(RT-PCR). RESULTS The result of HE showed that the scores of gastric mucosal lesions of the high-fat diet group and the Hp infection group were significantly higher than those of the control group(P<0.05);the score of gastric mucosal lesions of the high-fat diet + Hp infection group was significantly higher than that of the high-fat diet group and the Hp infection group(P<0.05). The levels of serotonin(5-HT), gastrin(Gas) and motilin(MTL) in gastric tissues and plasma of the high-fat diet group were significantly higher than those of the control group(P<0.05), while the levels of somatostatin(SS) and ghrelin(Grelin) of the high-fat diet group were significantly lower than those of the control group(P<0.05). The levels of 5-HT, Gas and MTL in gastric tissues and plasma of the high-fat diet plus Hp infection group were significantly higher than those of the high-fat diet group and the Hp infection group(P<0.05);while the levels of SS and Grelin of the high-fat diet plus Hp infection group were significantly lower than those of the high-fat diet group and the Hp infection group(P<0.05). The levels of tumor necrosis factor-ɑ(TNF-ɑ), interleukin(IL)-6 and IL-1β in gastric tissues of the high-fat diet

关 键 词：慢性胃炎 幽门螺杆菌 高脂饮食 胃肠激素 炎症反应 WNT/Β-CATENIN信号通路 

分 类 号：R573.3[医药卫生—消化系统]