草鱼源乳酸菌的分离鉴定及其生物学特性研究  

Isolation,identification and biological characteristics of lactobacillus from grass carp

作　　者：王楠 尹纪元 王英英[2] 李莹莹[2] 吴斯宇 石存斌[2] 李家豪 曹际振 王庆[2] WANG Nan;YIN Jiyuan;WANG Yingying;LI Yingying;WU Siyu;SHI Cunbin;LI Jiahao;CAO Jizhen;WANG Qing(Shanghai Ocean University/National Demonstration Center for Experimental Fisheries Science Education,Shanghai 201306,China;Pearl River Fisheries Research Institute,Chinese Academy of Fishery Sciences/Key Laboratory of Fishery Drug Development,Ministry of Agriculture and Rural Affairs/Key Laboratory of Aquatic Animal Immune Technology of Guangdong Province,Guangzhou 510380,China)

机构地区：[1]上海海洋大学/水产科学国家级实验教学示范中心,上海201306 [2]中国水产科学研究院珠江水产研究所/农业农村部渔药创制重点实验室/广东省水产动物免疫技术重点实验室,广东广州510380

出　　处：《南方水产科学》2021年第6期74-84,共11页South China Fisheries Science

基　　金：国家重点研发计划项目(2019YFD0900102);广东省促进经济高质量发展专项资金海洋经济发展项目(GDOE〔2019〕A29);中国水产科学研究院珠江水产研究所基本科研业务费(2019XN-001);财政部和农业农村部国家现代农业产业技术体系资助(CARS-45)。

摘　　要：随着水产养殖病害问题日益严重,抗生素和药物使用对环境造成的危害越来越大,开展绿色生态防控技术研究是实现水产健康养殖的重要途径。该研究从健康草鱼(Ctenopharyngodon idella)肠道分离获得8株乳酸菌(Lactobacillus),经生化反应和16S rRNA鉴定,均为植物乳杆菌(L.plantarum)。生物学特性评价结果表明植物乳杆菌分离株Y190430的发酵性能最好,且具有良好的抗生素敏感性;与植物乳杆菌标准株ATCC8014和商品乳酸菌相比较,分离株Y190430对酸、碱、盐、温度等环境胁迫具有更好的耐受性,同时具有更快的产酸速率,并可通过代谢产物抑制病原菌生长,体外抑菌试验表明分离株Y190430对常见水产病原菌的拮抗能力更强。As the problem of aquaculture diseases is growing,the harm of using antibiotics and drugs is more and more serious so the research on ecological control technology is an important way to achieve healthy aquaculture.In this study,eight lactobacillus strains were isolated from the intestinal tract of healthy grass carps(Ctenopharyngodon idella).The identification result indicates that all the eight strains were Lactobacillus plantarum.The results of biological characteristics evaluation show that L.plantarum strain Y190430 had a high growth rate,and it was sensitive to common antibiotics.In addition,compared with the standard strain and commercial lactobacillus,L.plantarum Y190430 represented better tolerance to environmental stress such as acid,alkali,salt and temper-ature.Besides,it had a higher acid production rate and inhibited the growth of pathogenic bacterias better.The results provide references for the research on ecological control of aquatic diseases.

关 键 词：乳酸菌 发酵性能 抗生素敏感性 生态防控 益生功能 

分 类 号：S917.1[农业科学—水产科学]