生物信息学分析DHX9在肝细胞癌中表达及临床意义  

Bioinformatics analysis of DHX9 expression in hepatocellular carcinoma and clinical significance

作　　者：袁晓峰[1] 徐雯[1] 闫凌 毕筱刚[1] 李明亮[1] Yuan Xiaofeng;Xu Wen;Yan Ling;Bi Xiaogang;Li Mingliang(General ICU,Lingnan Hospital of the Third Affiliated Hospital of Sun Yat-sen University,Guangzhou 510530,China)

机构地区：[1]中山大学附属第三医院岭南医院综合ICU,广州510530

出　　处：《中华肝脏外科手术学电子杂志》2021年第6期636-641,共6页Chinese Journal of Hepatic Surgery(Electronic Edition)

摘　　要：目的探讨DEAH盒解旋酶9(DHX9)在肝细胞癌(肝癌)早期诊断和治疗中的作用。方法利用GEPIA、Oncomine、The Human Protein Atlas在线平台从DNA、mRNA和蛋白质3个层面分析肝癌组织和正常肝组织中DHX9的表达情况。通过UALCAN、Kaplan-Meier Plotter在线平台分析DHX9与肝癌临床特征和预后的关系。cBioPortal在线平台分析肝癌中的DHX9基因突变情况,LinkedOmics、WebGestalt在线平台分析DHX9基因的生物学意义。STRING在线平台构建DHX9蛋白相互作用网络。结果GEPIA、Oncomine、The Human Protein Atlas在线平台分析结果显示,与正常肝组织相比,DHX9 mRNA、DHX9 DNA、DHX9蛋白质在肝癌中明显高表达。DHX9 mRNA表达与年龄、TP53突变、疾病分期和肿瘤分级有关。Kaplan-Meier Plotter在线平台分析显示,DHX9 mRNA低表达肝癌患者的总体生存、疾病特异性生存和无进展生存预后更好(HR=1.48,1.62,1.57;P<0.05)。DHX9基因突变发生率17%(63/370),大多数基因突变为扩增和mRNA增高。DHX9基因突变的表达水平与肿瘤组织学分级有关(P=0.017)。DHX9基因主要参与DNA修复、有丝分裂、DNA代谢、细胞周期调控、解螺旋酶活性、转录因子结合等活动。京都基因与基因组百科全书(KEGG)通路分析显示,DHX9基因在mRNA监测通路、RNA转运、酪氨酸代谢、过氧化物酶体增殖物激活受体(PPAR)信号通路等中富集。DHX9蛋白参与DNA修复途径,促进核糖体招募、翻译起始,细胞的生长和分裂等活动。结论DHX9基因主要参与DNA修复、RNA转运、细胞周期调控、PPAR信号通路等重要生物学过程。DHX9在肝癌组织中高表达,与肿瘤分级、分期有关,且低表达患者生存预后较好。Objective To investigate the role of DEAH-box helicase 9(DHX9)in the early diagnosis and treatment of hepatocellular carcinoma(HCC).Methods The expression profile of DHX9 in the HCC and normal liver tissues was analyzed from the DNA,mRNA and protein aspects on GEPIA,Oncomine and The Human Protein Atlas.The relationship between DHX9 and clinical features and prognosis of HCC patients was analyzed with UALCAN and Kaplan-Meier Plotter online analysis tools.DHX9 gene mutation in HCC was analyzed on cBioPortal.The biological significance of DHX9 gene was analyzed on LinkedOmics and WebGestalt.The DHX9 protein interaction network was constructed by STRING online analysis tool.Results The expression levels of DHX9 mRNA,DHX9 DNA and DHX9 protein in the HCC tissues were significantly higher compared with those in the normal liver tissues according to the analysis results of GEPIA,Oncomine and The Human Protein Atlas.The expression level of DHX9 mRNA was associated with age,TP53 mutation,disease staging and tumor grading.The overall survival,disease-specific survival and progression-free survival of HCC patients with low expression of DHX9 mRNA were better based on the analysis of Kaplan-Meier Plotter(HR=1.48,1.62,1.57;P<0.05).The mutation rate of DHX9 gene was 17%(63/370),and a majority of the mutations were amplification and mRNA increase.The expression level of DHX9 gene mutation was correlated with the histological grading of tumors(P=0.017).DHX9 gene was mainly involved in DNA repair,mitosis,DNA metabolism,cell cycle regulation,helicases activity and transcriptional factor-binding capability,etc.Kyoto Encyclopedia of Genes and Genomes(KEGG)signaling pathway analysis revealed that DHX9 gene was enriched in the mRNA monitoring pathway,RNA transport,tyrosine metabolism and peroxisome proliferators-activated receptor(PPAR)signaling pathway,etc.DHX9 protein participated in DNA repair pathway and promoted ribosome recruitment,initiation of translation,cell growth and division,etc.Conclusions DHX9 gene is mainly involved in

关 键 词：癌 肝细胞 计算生物学 早期诊断 DEAH盒解旋酶9 

分 类 号：R735.7[医药卫生—肿瘤]