Microbiota modulates gut immunity and promotes baculovirus infection in Helicoverpa armigera  

作　　者：Chuanfei Yuan Longsheng Xing Manli Wang Zhihong Hu Zhen Zou 

机构地区：[1]State Key Laboratory of Virology,Wuhan Institute of Virology,Chinese Academy of Sciences,Wuhan,430071,China [2]State Key Laboratory of Integrated Management of Pest Insects and Rodents,Institute of Zoology,Chinese Academy of Sciences,Beijing,100101,China [3]Key Laboratory of Tropical Translational Medicine of Ministry of Education,NHC Key Laboratory of Control of Tropical Diseases,School of Tropical Medicine and Laboratory Medicine,Hainan Medical University,Haikou,571199,China [4]Shenzhen Branch,Guangdong Laboratory for Lingnan Modern Agriculture,Genome Analysis Laboratory of the Ministry of Agriculture,Agricultural Genomics Institute at Shenzhen,Chinese Academy of Agricultural Sciences,Shenzhen,518120,China [5]CAS Center for Excellence in Biotic Interactions,University of Chinese Academy of Sciences,Beijing,100049,China

出　　处：《Insect Science》2021年第6期1766-1779,共14页昆虫科学（英文版）

基　　金：supported by grants from National Key Plan for Scientific Research and Development of China(2019YFC1200504);National Natural Science Foundation of China(grant numbers 31621061 and 31872298);Open Research Fund Program of State Key Laboratory of Integrated Management of Pest Insects and Rodents(Chinese IPM2015).

摘　　要：Baculoviruses are natural enemies of agricultural and forest insect pests and play an important role in biological pest control.Oral infection by baculovirus in the insect midgut is necessary for establishing systemic infection and eventually killing the insect.Since the insect midgut continuously encounters microbiota,the gut microbiota could affect baculovirus infection.Here,we demonstrated that gut microbiota modulates immune responses and promotes baculovirus infection in the cotton bollworm,Helicoverpa armigera.After oral infection,numerous host immunity-related genes including genes encoding Toll and immune deficiency(IMD)pathway components were upregulated in the midgut.Elimination of the gut microbiota significantly increased the resistance to viral infection in H.armigera.Quantitative real-time reverse transcription polymerase chain reaction and proteomic analysis showed that downregulation of the antiviral factor prophenoloxidase(PPO)could be mediated by microbiota during infection.It implied that midgut microbiota diminishes the expression of PPO to facilitate viral infection in H.armigera.Our findings revealed that the microbiota plays an important role in modulating the resistance of H.armigera to baculovirus infection,providing new insights in applying biopesticide.

关 键 词：BACULOVIRUS Helicoverpa armigera IMMUNITY midgut microbiota oral infection 

分 类 号：Q78[生物学—分子生物学]