高效液相色谱柱后衍生法测定啤酒中甲醛含量  

Determination of Formaldehyde in Beer by HPLC with Post-column Derivatization

作　　者：曾文祥 李珍妮 曾川 钟敏 ZENG Wenxiang;LI Zhenni;ZENG Chuan;ZHONG Min(Gongbei Customs Technology Center,Zhuhai 519000,China;Guangdong Pharmaceutical University,Zhongshan 528400,China)

机构地区：[1]拱北海关技术中心,广东珠海519000 [2]广东药科大学,广东中山528400

出　　处：《食品安全导刊》2021年第34期75-78,共4页China Food Safety Magazine

摘　　要：本文利用甲醛与乙酰丙酮及铵离子反应生产黄色产物原理,研究出高效液相色谱柱后衍生法测定啤酒中甲醛含量方法,考察了方法专属性、检出限、定量限、标准曲线线性范围、方法准确度、衍生温度及取样量因素,试验结果表明本方法检出限为0.2 mg/L,定量限为0.5 mg/L,甲醛上机浓度在0.01~5.0 mg/L范围线性良好,确定了衍生器反应温度为80℃,取样量为0.5 mL。本方法与GB/T 5009.49—2008相比,具有前处理简单,样品无需蒸馏,精密度准确度更高等优点。This article utilizes the theory of formaldehyde reacts with acetyl acetone and ammonium ions to produce yellow products,to establish a method for a determination of formaldehyde in beer by HPLC with post-column derivatization.After studying the factors of method specificity,accuracy,LOD,LOQ,standard curve linear range,post-column derivatization temperature and sampling volume,the experimental results showed that the LOD was 0.2 mg/L,the LOQ was 0.5 mg/L,the linearity of formaldehyde concentration was good in the range of 0.01~5.0 mg/L,the detection temperature was determined to be 80℃,and the sampling volume was 0.5 mL.Comparing with GB/T 5009.49—2008,this method has the advantages of simpler pretreatment,especially cutting out the step of distillation,and higher precision and accuracy.

关 键 词：啤酒 甲醛 高效液相色谱 柱后衍生 

分 类 号：O657.72[理学—分析化学] TS262.3[理学—化学]