猪粪便中丁酸梭菌的分离与鉴定  

Isolation and identification of Clostridium butyricum in swine manure

作　　者：冯腾柱 李市场[1] 龚明贵[1] 张自平 张亚娟 杜莹瑞 陈妍 张盎然 FENG Teng-zhu;LI Shi-chang;GONG Ming-gui;ZHANG Zi-ping;ZHANG Ya-juan;DU Ying-rui;CHEN Yan;ZHANG Ang-ran

机构地区：[1]河南科技大学食品与生物工程学院,河南洛阳471023

出　　处：《饲料研究》2021年第21期79-82,共4页Feed Research

基　　金：国家自然科学基金面上项目(项目编号:31870093);河南科技大学大学生训练计划项目(项目编号:2019179)。

摘　　要：试验以从河南省鄢陵县农户肉猪养殖场中采集的新鲜猪粪为样本,经过强化梭菌培养基(RCM)富集和TSN选择性琼脂培养基进行分离筛选和生理生化鉴定,得到2株菌株,分别命名为B1和B3。通过提取菌株DNA,并对16S rDNA序列进行扩增和测序,根据其同源性亲疏建立系统发育树,二者均被鉴定均为丁酸梭菌(Clostridium butyricum)。对2株菌的发酵特性进行测定,分析发酵液中菌浓度、pH值、还原糖、乙酸和丁酸含量随时间的变化。结果表明,B1和B3菌株在葡萄糖利用和乙酸的代谢水平上存在差异。Two strains were obtained from fresh swine manure samples collected from a farm in Yanling County, Henan Province after being enriched with reinforce Clostridium medium(RCM). The strains were screened and separated from trypticase sulfite neomycin(TSN) agar, and identified by physiological and biochemical identification. Two strains were named as B1 and B3, respectively. DNA of B1 and B3 were extracted, and the 16 S rDNA sequences were sequenced after amplified with PCR. Phylogenetic trees were established based on its homology and sparseness. The two strains were identified as Clostridium butyricum. The fermentation characteristics of the two strains were identified. The change of bacteria concentration, pH value, reducing sugar, acetate content and butyrate content followed by time were analyzed.The results showed a difference between C. butyricum B1 and B3 in glucose consumption and acetate accumulation.

关 键 词：丁酸梭菌 生理生化鉴定 16S rDNA鉴定 发酵特性 

分 类 号：S816.7[农业科学—饲料科学]