Peripheral nerve fibroblasts secrete neurotrophic factors to promote axon growth of motoneurons  

作　　者：Qian-Ru He Meng Cong Fan-Hui Yu Yu-Hua Ji Shu Yu Hai-Yan Shi Fei Ding 

机构地区：[1]Key Laboratory of Neuroregeneration of Jiangsu and Ministry of Education,Co-Innovation Center of Neuroregeneration,Jiangsu Clinical Medicine Center of Tissue Engineering and Nerve Injury Repair,Nantong University,Nantong,Jiangsu Province,China

出　　处：《Neural Regeneration Research》2022年第8期1833-1840,共8页中国神经再生研究（英文版）

基　　金：supported by the National Key Research and Development Program of China,No.2017YFA0104703(to FD);the National Natural Science Foundation of China(Major Program),No.92068112(to FD);Science and Technology Program of Nantong of China,No.JC2020035(to QRH);National Natural Science Foundation of China,Nos.31500927(to QRH)and 31870977(to HYS);the Priority Academic Program Development of Jiangsu High Education Institutions(PAPD)(to FD).

摘　　要：Peripheral nerve fibroblasts play a critical role in nerve development and regeneration.Our previous study found that peripheral nerve fibroblasts have different sensory and motor phenotypes.Fibroblasts of different phenotypes can guide the migration of Schwann cells to the same sensory or motor phenotype.In this study,we analyzed the different effects of peripheral nerve-derived fibroblasts and cardiac fibroblasts on motoneurons.Compared with cardiac fibroblasts,peripheral nerve fibroblasts greatly promoted motoneuron neurite outgrowth.Transcriptome analysis results identified 491 genes that were differentially expressed in peripheral nerve fibroblasts and cardiac fibroblasts.Among these,130 were significantly upregulated in peripheral nerve fibroblasts compared with cardiac fibroblasts.These genes may be involved in axon guidance and neuron projection.Three days after sciatic nerve transection in rats,peripheral nerve fibroblasts accumulated in the proximal and distal nerve stumps,and most expressed brain-derived neurotrophic factor.In vitro,brain-derived neurotrophic factor secreted from peripheral nerve fibroblasts increased the expression ofβ-actin and F-actin through the extracellular regulated protein kinase and serine/threonine kinase pathways,and enhanced motoneuron neurite outgrowth.These findings suggest that peripheral nerve fibroblasts and cardiac fibroblasts exhibit different patterns of gene expression.Peripheral nerve fibroblasts can promote motoneuron neurite outgrowth.

关 键 词：brain-derived neurotrophic factor differential gene expression F-ACTIN FIBROBLASTS MOTONEURONS mRNA sequencing neurite outgrowth peripheral nervous system Β-ACTIN 

分 类 号：R338[医药卫生—人体生理学]