N^(+)离子注入黑花生对OPC清除氧自由基能力影响的研究  

Study on the Effect of N^(+) Ion Implantation on Black Peanut on the Ability of OPC to Scavenge Oxygen Free Radical

作　　者：刘贵忠 LIU Gui-zhong(School of Physics and Electronic Information,Dezhou University,Dezhou Shandong 253023,China)

机构地区：[1]德州学院物理与电子信息学院,山东德州253023

出　　处：《德州学院学报》2021年第6期79-84,共6页Journal of Dezhou University

基　　金：山东省功能大分子生物物理重点实验室专项课题(13zx10).

摘　　要：为研究N^(+)离子注入黑花生对Oligomeric Proaflth Cyaflidin(OPC)清除氧自由基能力的影响,将不同能量剂量组合的N^(+)离子注入黑花生,种植传代后提取OPC,采用紫外-可见分光光度仪和荧光分光光度计分别对不同OPC与不同浓度H_(2)O_(2)结合后的光谱进行测量分析.由Lineweaver-Burk方程计算出结合位点数n在0.1—1.56之间;静态猝灭常数KA在10.59—31.94之间.N^(+)离子注入黑花生对OPC的结合位点数和猝灭常数有显著影响,为离子注入选育黑花生良种提供有益参考.In order to study the effect of N^(+) ion implantation into black peanuts on the ability of OPC to scavenge oxygen free radicals,N^(+) ions with different energy dose combinations were implanted into black peanuts,and OPC was extracted after planting and passage,using ultraviolet-visible spectrophotometer and fluorescence spectrophotometer respectively The spectra of different OPCs combined with different concentrations of H_(2)O_(2) were measured and analyzed.According to the Lineweaver-Burk equation,the number of binding sites n is between 0.1 and 1.56;the static quenching constant KA is between 10.59 and 31.94.N^(+) ion implantation of black peanut has a significant effect on the number of OPC binding sites and quenching constant,which provides a useful reference for ion implantation to breed black peanut varieties.

关 键 词：离子注入 低聚原花青素 结合位点数 静态猝灭常数 抗氧化率 

分 类 号：S565.2[农业科学—作物学]