不同酶对巫山淫羊藿中淫羊藿苷和朝藿定C含量的影响及抑菌活性研究  

Effects of Different Enzymes on Contents of Icariin and Epimedin C from Epimedium wushanense Ying and Bacteriostatic Activity

作　　者：谢娟平 常永宏[2] 强世喜 刘春阳 陈嘉悦 XIE Juanping;CHANG Yonghong;QIANG Shixi;LIU Chunyang;CHEN Jiayue(College of Medicine,Ankang University,Ankang 725000,China;Qinba Chinese Medicine Resources R & D Center,Ankang University,Ankang 725000,China)

机构地区：[1]安康学院医学院,陕西安康725000 [2]安康学院秦巴中药资源研发中心,陕西安康725000

出　　处：《化学与生物工程》2021年第12期30-33,36,共5页Chemistry & Bioengineering

基　　金：陕西省科技厅项目(2019ZDLSF04-02,2019TSLSF02-03);校企项目(2019AYHX058);大学生创新创业项目(S201911397014)。

摘　　要：在巫山淫羊藿提取物中分别加入α-淀粉酶、果胶酶、纤维素酶进行酶解,经大孔树脂纯化后采用HPLC法测定巫山淫羊藿中淫羊藿苷和朝藿定C含量;并初步研究了巫山淫羊藿提取物对金黄色葡萄球菌和大肠杆菌的抑菌活性。结果表明,巫山淫羊藿提取物在经过酶解和纯化后,淫羊藿苷和朝藿定C的含量均明显增加,其中果胶酶对两者含量的影响最大,巫山淫羊藿提取物在经过果胶酶酶解和大孔树脂纯化后,淫羊藿苷和朝藿定C的含量分别增至11.65%、31.79%;巫山淫羊藿提取物及果胶酶酶解物对金黄色葡萄球菌和大肠杆菌均有较好的抑菌效果,但未酶解的巫山淫羊藿提取物的抑菌活性优于酶解物和纯化物。We determined the contents of icariin and epimedin C in Epimedium wushanense Ying through content determination by HPLC method after enzymolysis withα-amylase,pectinase,and cellulase and purification by macroporous resin.Moreover,we preliminarily studied the bacteriostatic activity of Epimedium wushanense Ying extract against Staphylococcus aureus and Escherichia coli.The results show that after enzymolysis and purification of Epimedium wushanense Ying extract,the contents of icariin and epimedin C significantly increase to 11.65%and 31.79%,respectively,and pectinase has the greatest effect on their contents.The Epimedium wushanense Ying extract and pectinase hydrolysate have good bacteriostatic effects on Staphylococcus aureus and Escherichia coli,but the bacteriostatic activity of Epimedium wushanense Ying extract without enzymolysis is superior to that of the hydrolysate and the purified product.

关 键 词：巫山淫羊藿 酶解 纯化 淫羊藿苷 朝藿定C 抑菌活性 

分 类 号：Q946.5[生物学—植物学] R284[医药卫生—中药学]