温度对黑豆皮活性成分、抗氧化和抗肿瘤活性的影响  

Effects of Temperature on Active Components,Antioxidant Activity,and Anti-Tumor Activity of Black Bean Hulls

作　　者：胡懿化 鲁静武 何嘉琦 王星滟 张武霞 李鹏[1] 张永坡[1] 高春艳[1] 岳爱琴[2] 杜维俊[2] 赵晋忠[1] HU Yihua;LU Jingwu;HE Jiaqi;WANG Xingyan;ZHANG Wuxia;LI Peng;ZHANG Yongpo;GAO Chunyan;YUE Aiqin;DU Weijun;ZHAO Jinzhong(Department of Basic Sciences,Shanxi Agricultural University,Taigu 030801,China;College of Agriculture,Shanxi Agricultural University,Taigu 030801,China)

机构地区：[1]山西农业大学基础部,山西太谷030801 [2]山西农业大学农学院,山西太谷030801

出　　处：《化学与生物工程》2021年第12期54-59,共6页Chemistry & Bioengineering

基　　金：山西省重点研发计划项目(201903D211006);国家自然科学基金青年基金项目(31800678);山西省高校创新基金项目(2021L146);山西农业大学育种工程项目(YZGC096);山西省研究生教育创新项目(2020SY198)。

摘　　要：在不同温度下用蒸馏水浸提黑豆皮,测定了黑豆皮提取物中总黄酮、总酚、花青素、碳水化合物、蛋白质、糖醛酸等6种活性成分的含量,并体外评估了其抗氧化活性和抗肿瘤活性。结果表明,90℃浸提时,黑豆皮提取物中的总黄酮、总酚、蛋白质、糖醛酸含量最高,分别为(11.555±0.160)%、(1.454±0.070)%、(13.096±0.600)%、(21.587±1.570)%;花青素含量在80℃浸提时达到最高,为(0.199±0.003)%;而碳水化合物的含量则在100℃浸提时达到最高,为(65.566±1.448)%。随着黑豆皮提取物浓度的增加,其总还原能力和对DPPH自由基的清除能力逐渐增强且呈线性关系;黑豆皮提取物具有抑制肿瘤细胞增殖的作用,能够有效抑制人胃腺癌细胞株(BGC-823)、宫颈癌细胞株(HeLa)和人大细胞肺癌细胞株(NCI-H460)的增殖且呈浓度依赖关系。表明黑豆皮提取物具有成为功能性保健品的潜能,为其产业化开发奠定了重要基础。In this paper,we obtained the black bean hulls extracts by water extraction at different temperatures.Moreover,we determined the contents of six active components in the black bean hulls extracts,including total flavonoids,total phenols,anthocyanin,carbohydrate,protein,and uronic acid.Furthermore,we evaluated their antioxidant activity and anti-tumor activity in vitro.The results show that the contents of total flavonoids,total phenols,protein,and uronic acid in the black bean hulls extracts by water extraction at 90℃are the highest of(11.555±0.160)%,(1.454±0.070)%,(13.096±0.600)%,and(21.587±1.570)%,respectively;the content of anthocyanin reaches the highest of(0.199±0.003)%by water extraction at 80℃;the content of carbohydrate reaches the highest of(65.566±1.448)%by water extraction at 100℃.With the increase of the concentration of the black bean hulls extracts,the total reduction ability and DPPH free radical scavenging ability increase gradually and show a linear relationship.The black bean hulls extracts can inhibit the proliferation of tumor cells,and can effectively inhibit the proliferation of human gastric adenocarcinoma cell(BGC-823),cervical cancer cell(HeLa),and human lung cancer cell(NCI-H460)in a concentration dependent manner.The study shows that the black bean hulls extracts has the potential to become functional health products,which lays an important foundation for the industrial development.

关 键 词：黑豆皮 活性成分 热稳定性 抗氧化活性 抗肿瘤活性 温度 

分 类 号：TQ914[化学工程] R284[医药卫生—中药学]