珊瑚藻多糖酶法提取工艺优化及其在卷烟中的应用  

Optimization of Enzymatic Extraction Process of Coralline Polysaccharides and Its Application in Cigarette

作　　者：张峰 谢全灵 余志强 操晓亮 张怡评 赵敏 洪专 郭洪辉 陈晖 洪祖灿 ZHANG Feng;XIE Quanling;YU Zhiqiang;CAO Xiaoliang;ZHANG Yiping;ZHAO Min;HONG Zhuan;GUO Honghui;CHEN Hui;HONG Zucan(Technical Center of China Tobacco Fujian Industrial Co.,Ltd.,Xiamen 361022,China;Third Institute of Oceanography,Ministry of Natural Resources,Xiamen 361005,China;Fujian Collaborative Innovation Center for Exploitation and Utilization of Marine Biological Resources,Xiamen 361005,China)

机构地区：[1]福建中烟工业有限责任公司技术中心,厦门361022 [2]自然资源部第三海洋研究所,厦门361005 [3]福建省海洋生物资源开发利用协同创新中心,厦门361005

出　　处：《中国烟草科学》2021年第6期79-85,共7页Chinese Tobacco Science

基　　金：福建中烟工业有限责任公司重点科技项目(FJYKJJK2020020);厦门市科技计划项目(3502Z20203088)。

摘　　要：为了开发天然海藻多糖烟用保润剂,采用酶解法从珊瑚藻中提取多糖,首先考察5种酶对多糖提取量以及卷烟吸食品质的影响;在优选木聚糖酶的基础上,进一步通过单因素试验考察酶解温度、酶解时间、酶添加量、柠檬酸浓度等提取工艺条件对多糖提取量的影响;最后设计4因素3水平正交试验优化珊瑚藻多糖的酶法提取工艺。结果表明,最优酶法提取工艺条件为:木聚糖酶添加量为0.8%(m/m),酶解温度50℃,酶解时间3 h,柠檬酸浓度为0.0625 g/L,珊瑚藻多糖提取量为(661.1±7.8)mg/g。感官分析评价结果表明,添加适量的珊瑚藻多糖具有显著改善卷烟吸食品质的效果。The aim of this study was to develop natural polysaccharide humectant for cigarette.The method of enzymatic hydrolysis was used to extract polysaccharides from coralline algae.Firstly,the effects of five kinds of enzymes on the extraction amount of polysaccharides and their corresponding sensory evaluation of smoking quality were studied.After selecting xylanase as the optimal enzyme,the single-factor experiments were carried out to systematically investigate the influences of enzymolysis temperature,enzymolysis time,enzyme content,and citric acid concentration on the extraction amount of polysaccharides.Finally,four factors and three levels orthogonal experiments were designed to optimize the enzymatic extraction process.The results showed that the optimum conditions of the enzymatic extraction process were as follows:xylanase dosage 0.8%(m/m),enzymolysis temperature 50℃,enzymolysis time 3 h,citric acid concentration 0.0625 g/L.Under the optimal enzymatic extraction process,the extraction amount of coralline polysaccharides reached(661.1±7.8)mg/g.The results of the sensory evaluation showed that the smoking qualities of cigarettes were significantly improved with the addition of an appropriate amount of coralline polysaccharides.

关 键 词：珊瑚藻 酶法提取 木聚糖酶 多糖 卷烟 

分 类 号：TS426[农业科学—烟草工业]