绿茶提取物EGCG预防龋病的动物实验研究  

Animal experiment study on green tea extract EGCG to prevent dental caries

作　　者：李芳 刘亚军 刘泽婷 陈永翔 王烈成 王一君[3] 王元银[1] Li Fang;Liu Yajun;Liu Zeting(Stomatological College of Anhui Medical University,Hefei 230032)

机构地区：[1]安徽医科大学口腔医学院,合肥230032 [2]安徽医科大学基础医学院生理学教研室,合肥230032 [3]安徽农业大学茶与食品科技学院,合肥230036

出　　处：《安徽医科大学学报》2021年第12期1903-1907,共5页Acta Universitatis Medicinalis Anhui

基　　金：国家自然科学基金(编号:81271162);安徽省人口健康领域项目(编号:201904a07020062);安徽农业大学茶树生物学与资源利用国家重点实验室开放基金资助项目(编号:SKLTOF20160110)。

摘　　要：目的通过大鼠龋齿模型探讨表没食子儿茶素没食子酸酯(EGCG)对龋病的预防效果,为天然药物EGCG预防龋病的临床研究提供试验和理论依据。方法选择SD大鼠24只,随机分成EGCG、氟化钠(NaF)、醋酸氯己定(CHX)及蒸馏水(对照)组,每组6只。接种变形链球菌(S.mutans)标准菌株并给予致龋饲料2000#和5%蔗糖水,分别用浓度为最小抑菌浓度(MIC)的EGCG、250 mg/L NaF溶液、0.12%CHX溶液及蒸馏水对大鼠口腔进行冲洗涂药。连续处理6周后处死大鼠,收集大鼠颌骨,Keyes记分法分析,评估大鼠上颌磨牙患龋情况。结果经龋齿Keyes记分显示与对照组相比,各组药物对大鼠龋齿的形成和严重程度均有不同程度的抑制效果,其中EGCG组显示对大鼠上颌窝沟面龋的釉质龋(P<0.01)及牙本质龋均有有较好的防治效果,对牙本质深龋的防治效果更好(P<0.001)。但对牙本质浅、中龋的防治效果低于CHX组。结论MIC浓度的EGCG降低龋齿的发生率和严重程度,对大鼠龋损发生具有较好的抑制作用。Objective To explore the preventive effect of epigallocatechin gallate(EGCG)on dental caries through the rat dental caries model,and provide experimental and theoretical basis for clinical research of natural medicine EGCG on preventing caries.Methods Twenty-four SD rats were selected and randomly divided into EGCG group,sodium fluoride(NaF)group,chlorhexidine acetate(CHX)group and distilled water(control)group,each with 6 rats.The rats were inoculated with standard strain of streptococcus mutans(S.mutans)and given cariogenic feed 2000#and 5%sucrose solution.The oral cavity of rats was rinsed and coated with minimum inhibitory concentration(MIC)EGCG,250 mg/L NaF solution,0.12%CHX solution and distilled water,respectively.After 6 weeks of continuous treatment,the rats were sacrificed.The jaws of the rats were collected and analyzed by Keyes score method to evaluate the caries of the rats′maxillary molars.Results The Keyes scores of caries showed that compared with the control group,drugs in each group had different degrees of inhibition effect on the formation and severity of caries in rats.Among them,the EGCG group had better effect on enamel caries(P<0.01)and dentin caries,and had much better effect on dentin deep caries(P<0.001)of maxillary fossa and fissure caries in rats.However,the prevention and treatment effect of superficial and middle dental caries was lower than that of CHX group.Conclusion EGCG with MIC concentration reduces the incidence and severity of dental caries,and has a significant inhibitory effect on the occurrence of dental caries in rats.

关 键 词：表没食子儿茶素没食子酸酯 EGCG 变形链球菌 SD大鼠 龋病 

分 类 号：R781.1[医药卫生—口腔医学]