亚健康状态人群肠道菌群结构明显失调:基于某医院150例护理人员分析  

Individuals with sub-health status have obviously unbalanced structure of the intestinal flora:analysis of 150 nursing staff members

作　　者：张星星[1,2] 蔡文文 廖生武 何璇昱[1] 杨秋玉 白杨 阮伟清[1] ZHANG Xingxing;CAI Wenwen;LIAO Shengwu;HE Xuanyu;YANG Qiuyu;BAI Yang;RUAN Weiqing(Huiqiao Medical Center,Nanfang Hospital,Southern Medical University,Guangzhou 510515,China;School of Nursing,Southern Medical University,Guangzhou 510515,China;Outpatient and Emergency Area,Nanfang Hospital,Southern Medical University,Guangzhou 510515,China;Department of Gastroenterology,Nanfang Hospital,Southern Medical University,Guangzhou 510515,China)

机构地区：[1]南方医科大学南方医院惠侨医疗中心,广东广州510515 [2]南方医科大学护理学院,广东广州510515 [3]南方医科大学南方医院门急诊片区,广东广州510515 [4]南方医科大学南方医院消化内科,广东广州510515

出　　处：《南方医科大学学报》2021年第12期1870-1876,共7页Journal of Southern Medical University

基　　金：广东省科技计划项目(2019A141405048);广州市科技计划项目(202103000037)。

摘　　要：目的探索亚健康状态人群(SHS)和健康人群之间肠道菌群是否存在差异。方法于2020年11月~2021年5月选取南方医科大学南方医院工作的护理人员150例作为研究对象,其中亚健康人群75例为亚健康组(SHS组),选择75例同期健康人群作为对照组(H组)。针对16S rRNA基因的V3-V4区采用高通量测序技术,对测序结果进行多样性和物种组成差异分析。结果α多样性分析显示,Chao1指数在SHS组和H组间差异无统计学意义(P=0.619),SHS组的Shannon指数升高且2组之间差异有统计学意义(P<0.001);β多样性分析显示,2组间肠道菌群组成结构上差异具有统计学意义(R=0.227,P=0.001);在门水平上2组均主要由拟杆菌门(Bacteroidota)、厚壁菌门(Firmicutes)、放线菌门(Actinobacteriota)组成;在属水平均主要由普雷沃菌属(Prevotella)、拟杆菌属(Bacteroides)、布劳特氏菌属(Blautia)、栖粪杆菌属(Faecalibacterium)组成;物种差异分析表明,2组在4个门3个纲3个目3个科3个属的相对丰度差异有统计学意义(P<0.05)。结论与健康人群相比,亚健康状态人群肠道菌群结构明显失衡。Objective To explore the difference in intestinal flora composition between individuals with sub-health status and healthy subjects.Methods From November,2020 to May,2021,a total of 150 nursing staff members in Nanfang Hospital were selected for this study,including 75 participants with sub-health status(SHS group)and 75 healthy participants(control group).Fecal samples were collected from all the participants for analysis of the diversity and species composition of the intestinal flora using high-throughput sequencing for V3-V4 region of 16S rRNA gene.Results The results of α diversity analysis showed no significant difference in Chao1 index between the two groups(P=0.619),but the Shannon index was significantly higher in SHS group than in the control group(P<0.001).The results ofβdiversity analysis showed significant differences in the community structure between the SHS group and the control group(R=0.227,P=0.001).At the phylum level,the intestinal flora in the two groups were composed mainly of Bacteroidota,Firmicutes and Actinobacteriata,and of Prevotella,Bacteroides,Blautia and Faecalibacterium at the genus level.Species difference analysis identified significant differences in the relative abundance between the two groups in 4 phyla,3 classes,3 orders,3 families and 3 genera(P<0.05).Conclusion Compared with healthy subjects,the individuals with sub-health status have obviously unbalanced structure of the intestinal flora.

关 键 词：亚健康状态 肠道菌群 16S rRNA 慢性疲劳综合征 

分 类 号：R57[医药卫生—消化系统]