慢性粒细胞白血病患者血液指标检查与人类白细胞抗原基因的相关性  

Correlation between blood index and human leukocyte antigen gene in patients with chronic myelogenous leukemia

作　　者：李露 LI Lu(Department of Laboratory Medicine,Liaoning Health Industry Group Iron Coal General Hospital,Tieling 112700,China)

机构地区：[1]辽宁省健康产业集团铁煤总医院检验科,112700

出　　处：《中国实用医药》2021年第35期21-24,共4页China Practical Medicine

摘　　要：目的探讨慢性粒细胞白血病(CML)患者血液指标检查与人类白细胞抗原(HLA)基因的相关性。方法选择64例慢性粒细胞白血病患者作为白血病组,另选择64例同期在本院体检的健康人群作为对照组。调查两组的血液指标,检测HLA基因表达、突变情况。对比两组HLA-A、HLA-B、HLA-DRB1等位基因突变率,白细胞计数(WBC)、红细胞计数(RBC)、血小板计数(PLT)异常率,红细胞参数[平均红细胞血红蛋白含量(MCH)、平均红细胞血红蛋白浓度(MCHC)、红细胞分布宽度(RDW)、平均红细胞体积(MCV)]。并分析HLA等位基因突变率与各指标的相关性,以及慢性粒细胞白血病发生的影响因素。结果两组共检出13个HLA-A基因、22个HLA-B基因和11个HLA-DRB1基因,呈现较高的基因多态性。白血病组的HLA-A、HLA-B、HLA-DRB1等位基因突变率分别为12.5%、18.8%和10.9%,高于对照组的1.6%、4.7%和0,差异有统计学意义(P<0.05)。白血病组的WBC、RBC、PLT异常率分别为96.9%(62/64)、53.1%(34/64)和70.3%(45/64),高于对照组的1.6%(1/64)、3.1%(2/64)、1.6%(1/64),差异具有统计学意义(P<0.05)。白血病组的MCH(28.48±2.11)pg、MCHC(319.87±13.33)g/L低于对照组的(30.87±1.65)pg、(334.01±8.99)g/L,RDW(18.34±2.22)%、MCV(93.22±2.18)fl高于对照组的(13.28±0.83)%、(91.73±3.28)fl,差异有统计学意义(P<0.05)。白血病组患者相关性分析显示,HLA等位基因突变率与WBC、RBC、PLT异常率和RDW呈正相关,与MCHC呈负相关(P<0.05)。Logistic回归分析显示,HLA等位基因突变率、RDW及WBC、PLT异常率为导致慢性粒细胞白血病发生的影响因素(P<0.05)。结论慢性粒细胞白血病多伴有血液指标检查异常与HLA等位基因突变,两者存在相关性。Objective To discuss the correlation between blood index and human leukocyte antigen(HLA)gene in patients with chronic myelogenous leukemia(CML).Methods 64 cases of patients with chronic myelogenous leukemia were selected as the leukemia group,and 64 cases of healthy people who received physical examination in this hospital during the same period were selected as the control group.The blood indexes of the two groups were investigated, and the HLA gene expression and mutation were detected. Comparison was made on mutation rates of HLA-A, HLA-B and HLA-DRB1 alleles, abnormal rates of white blood cell count (WBC), red blood cell count (RBC) and platelet count (PLT), red blood cell parameters[ mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), red-blood-cell distribution width (RDW), mean corpuscular volume (MCV)]. The correlation between HLA allele mutation rate and various indicators, as well as the influencing factors of chronic myelogenous leukemia were analyzed. Results A total of 13 HLA-A genes, 22 HLA-B genes and 11 HLA-DRB1 genes were detected in the two groups, showing high gene polymorphism. The mutation rates of HLA-A, HLA-B and HLA-DRB1 alleles in the leukemia group were 12.5%, 18.8% and 10.9%, respectively, which were higher than 1.6%, 4.7% and 0 in the control group, and the differences were statistically significant (P<0.05). The abnormal rates of WBC, RBC, and PLT in the leukemia group were 96.9%(62/64), 53.1%(34/64), and 70.3%(45/64), respectively, which were higher than 1.6%(1/64), 3.1%(2/64) and 1.6%(1/64) in the control group, and the differences were statistically significant (P<0.05). The MCH (28.48±2.11) pg and MCHC (319.87±13.33) g/L in the leukemia group were lower than (30.87±1.65) pg and (334.01±8.99) g/L in the control group, and the RDW (18.34±2.22)% and MCV (93.22±2.18) fl were higher than (13.28±0.83)% and (91.73±3.28) fl in the control group. All the differences were statistically significant (P<0.05). Correlation analysis of patients in the le

关 键 词：慢性粒细胞白血病 血液指标 人类白细胞抗原 基因突变 相关性 

分 类 号：R733.72[医药卫生—肿瘤]