基于网络药理学及分子对接探讨二至天癸方治疗女性不孕症的作用机制  

Mechanism of Erzhi Tiangui prescription in treating female infertility based on network pharmacology

作　　者：李然 郭颖[2] LI Ran;GUO Ying(Graduate School,Shandong University of Traditional Chinese Medicine,First Affiliated Hospital of Shandong University of Traditional Chinese Medicine,Jinan 250014,China;Dept of Gynecology,Reproductive Center,First Affiliated Hospital of Shandong University of Traditional Chinese Medicine,Jinan 250014,China)

机构地区：[1]山东中医药大学研究生院,山东济南250014 [2]山东中医药大学附属医院生殖中心妇二科,山东济南250014

出　　处：《中国药理学通报》2022年第1期126-133,共8页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金青年基金项目(No 81804130)。

摘　　要：目的从网络药理学的层面观察二至天癸方对于女性不孕症的干预机制,为高龄女性不孕症的中医药治疗提供系统生物学信息和基础。方法基于网络药理学,检索二至天癸方的主要化学成分及作用靶点,分析其生物学通路,并采用分子对接技术预测其结合活性。结果筛选出活性成分159种,对应靶点283个,女性不孕症相关疾病靶点1078个,交集靶点共104个,主要涉及PTGS2、ESR1、HSP90AA1、AR、NOS2、PPARG、NCOA2等关键靶蛋白。生物学通路主要作用于PI3K-Akt信号通路、MAPK信号通路等。结论该研究初步探讨了二至天癸方调治女性不孕症的多途径、多成分、多靶点的作用机理,其主要活性成分与Mfn-1、Mfn-2较好结合,可能对高龄女性不孕症发挥治疗作用。Aim To observe the intervention mechanism of Erzhi Tiangui prescription on female infertility from the perspective of network pharmacology, so as to provide systematic biological information and basis for the treatment of infertility in elderly women with traditional Chinese medicine. Methods Based on network pharmacology, the main chemical constituents and action targets of Erzhi Tiangui prescription were searched, its biological pathway was analyzed, and its binding activity was predicted by molecular docking technique. Results A total of 159 active components were screened, with 283 corresponding targets, 1 078 female infertility related disease targets and 104 intersection targets, mainly involving key target proteins such as PTGS2, ESR1, HSP90 AA1, AR, NOS2, PPARG, NCOA2 and so on. The biological pathways mainly acted on PI3 K-Akt signaling pathway, MAPK signaling pathway and so on. Conclusions The study preliminarily explores the mechanism of Erzhi Tiangui prescription in regulating female infertility through multi-ways, multi-components and multi-targets, and its main active components are well combined with Mfn-1 and Mfn-2, which may play a therapeutic role in the treatment of infertility in elderly women.

关 键 词：二至天癸方 女性不孕症 网络药理学 分子对接 靶点预测 信号通路 

分 类 号：R223.14[医药卫生—中医基础理论] R289.5[医药卫生—中医学]