耐硒菌株 Lysinibacillus fusiformis D1的鉴定及清除自由基能力研究  

Screening and identification of selenium-tolerant Lysinibacillus fusiformis D1 and scavenging activity on oxygen free radicals

作　　者：段玉桦 余雍和 杨锐 王一丹 周欣 王璋倩 DUAN Yuhua;YU Yonghe;YANG Rui;WANG Yidan;ZHOU Xin;WANG Zhangqian(College of Life Science and Technology,Wuhan Polytechnic University,Wuhan 430023,China;School of Modern Industry for Selenium Science and Engineering,Wuhan Polytechnic University,Wuhan 430040,China;National R&D Center for Se-Rich Agricultural Products Processing,Wuhan Polytechnic University,Wuhan 430040,China)

机构地区：[1]武汉轻工大学生命科学与技术学院,湖北武汉430023 [2]武汉轻工大学硒科学与工程现代产业学院,湖北武汉430040 [3]武汉轻工大学,国家富硒农产品加工技术研发专业中心,湖北武汉430040

出　　处：《食品与发酵工业》2021年第24期77-82,共6页Food and Fermentation Industries

基　　金：国家自然科学基金项目(31900011);武汉轻工大学科研项目资助(2019y05);武汉轻工大学大学生科研项目(xsky2020028);湖北省“楚天学者计划”人才项目。

摘　　要：硒污染土壤对生态环境和人体健康存在危害,国内利用微生物修复高硒土壤污染研究较少。从江汉平原土壤中以耐硒能力筛选出1株高耐硒菌株,对该菌株进行鉴定、生长动力学分析和纳米硒表征,并对其清除自由基活性进行评价。结果表明,该菌株为纺锤形赖氨酸芽孢杆菌,对亚硒酸钠最高耐受性为35 mg/mL。纳米硒粒径为(306.20±1.64)nm。菌株次生代谢产物有一定DPPH自由基清除能力[(66.38±0.67)%]和较强ABTS阳离子自由基清除能力[(99.94±0.08)%],该菌株制备纳米硒有一定羟自由基清除能力[(59.81±5.09)%]。该菌株可为富硒土壤污染的生物修复提供参考,其次生代谢产物中清除自由基物质发掘有待深入研究。Selenium-contaminated soil may be harmful to the ecological environment and human health.However,there are few studies on the use of microorganisms as a remedy in China.A high selenium tolerance strain was isolated from the soil of the Jianghan Plain.The strain was identified,its growth kinetics were analyzed,its nano-selenium was characterized,and its free radical scavenging activity was evaluated.The results showed that strain D1 was Lysinibacillus fusiformis and the highest tolerance to sodium selenite was 35 mg/mL.The particle size of nano-selenium was(306.20±1.64)nm.The secondary metabolites of Lysinibacillus fusiformis D1 had certain DPPH radical scavenging ability(66.38±0.67)%and strong ABTS radical scavenging ability(99.94±0.08)%.The nano-selenium produced by this strain had certain hydroxyl radical scavenging ability,which was(59.81±5.09)%.This strain can provide a reference for the bioremediation of selenium-rich soils,and the scavenging free radicals in its secondary metabolites need to be further studied.

关 键 词：纺锤形赖氨酸芽孢杆菌 耐硒 纳米硒 生物修复 自由基 

分 类 号：X172[环境科学与工程—环境科学]